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Literature summary for 1.2.1.13 extracted from
- Subunit structure and activity of glyceraldehyde-3-phosphate dehydrogenase from spinach chloroplasts (1978), Biochim. Biophys. Acta, 522, 19-31.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetate | preincubation with glyceraldehyde-phosphate, 10fold molar excess over iodoacetate, prevents inactivation | Spinacia oleracea |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Spinacia oleracea |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 43000 + x * 37000, SDS-PAGE | Spinacia oleracea |
| 43000 | - |
x * 43000 + x * 37000, SDS-PAGE | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.92 | - |
NAD+-dependent activity | Spinacia oleracea |
| 15.4 | - |
NADP+-dependent activity | Spinacia oleracea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 8.5, protein concentration 2.5 mg/ml, apoprotein, complete loss of activity after 48 h | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Spinacia oleracea | 3-phospho-D-glyceroyl phosphate + NADH | - |
r | |
| D-glyceraldehyde 3-phosphate + phosphate + NADP+ | - |
Spinacia oleracea | 3-phospho-D-glyceroyl phosphate + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000 + x * 37000, SDS-PAGE | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.8 | both directions | Spinacia oleracea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | cofactor | Spinacia oleracea | |
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Release 2023.1 (January 2023)
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