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Literature summary for 1.2.1.13 extracted from

  • Hensel, R.; Laumann, S.; Lang, J.; Heumann, H.; Lottspeich, F.
    Characterization of two D-glyceraldehyde-3-phosphate dehydrogenases from the extremely thermophilic archaebacterium Thermoproteus tenax (1987), Eur. J. Biochem., 170, 325-333.
    View publication on PubMed

General Stability

General Stability Organism
the enzyme is stabilized by glycerol, several salts, especially sodium or potassium phosphate Thermoproteus tenax

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
NADP+
-
Thermoproteus tenax
30
-
arsenate
-
Thermoproteus tenax
50
-
phosphate
-
Thermoproteus tenax

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
x * 39000, SDS-PAGE Thermoproteus tenax
39000
-
4 * 39000, SDS-PAGE Thermoproteus tenax
210000
-
gel filtration Thermoproteus tenax

Organism

Organism UniProt Comment Textmining
Thermoproteus tenax
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17
-
-
Thermoproteus tenax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + arsenate + NADP+
-
Thermoproteus tenax 3-phospho-D-glyceroyl arsenate + NADPH
-
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
Thermoproteus tenax 3-phospho-D-glyceroyl phosphate + NADPH
-
?

Subunits

Subunits Comment Organism
? x * 39000, SDS-PAGE Thermoproteus tenax
tetramer 4 * 39000, SDS-PAGE Thermoproteus tenax

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
96
-
half-life: 130 min Thermoproteus tenax
100
-
half-life: 35 min Thermoproteus tenax

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
oxidation of D-glyceraldehyde-3-phosphate Thermoproteus tenax
8
-
reduction of 3-phospho-D-glyceroyl phosphate Thermoproteus tenax

Cofactor

Cofactor Comment Organism Structure
NADP+ cofactor Thermoproteus tenax