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Literature summary for 1.2.1.12 extracted from

  • Barinova, K.V.; Eldarov, M.A.; Khomyakova, E.V.; Muronetz, V.I.; Schmalhausen, E.V.
    Isolation of recombinant human untagged glyceraldehyde-3-phosphate dehydrogenase from E.coli producer strain (2017), Protein Expr. Purif., 137, 1-6 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of untagged enzyme in Escherichia coli strain Rosetta 2 (DE3) Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Homo sapiens
-
3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P04406
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant untagged enzyme partially from Escherichia coli strain Rosetta 2 (DE3) by ammonium sulfate fractionation and precipitation in cyrstals, followed by desalting gel filtration, to homogeneity, method evaluation Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
after 6 months of storage, the specific activity of hGAPDH decreases to 10-15% of the original value due to the reversible oxidation of the enzyme. Reactivation of the enzyme by dissolving in 10 mM potassium phosphate, pH 7.4, and 5 mM DTT, to 1 mg/ml concentration, 2-4 h of incubation at 20-25°C, the specific activity of hGAPDH increases 5-7-fold. Alternatively, the enzyme can be dialyzed against required buffer solution containing 5 mM DTT Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
neuroblastoma cell
-
Homo sapiens
-
SH-SY5Y cell
-
Homo sapiens
-

Storage Stability

Storage Stability Organism
after 6 months of storage, the specific activity of hGAPDH decreases to 10-15% of the original value due to the reversible oxidation of the enzyme. Reactivation of the enzyme by dissolving in 10 mM potassium phosphate, pH 7.4, and 5 mM DTT, to 1 mg/ml concentration, 2-4 h of incubation at 20-25°C, the specific activity of hGAPDH increases 5-7-fold. Alternatively, the enzyme can be dialyzed against required buffer solution containing 5 mM DTT Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Homo sapiens 3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Subunits

Subunits Comment Organism
More human GAPDH identified by peptide mass fingerprinting and mass spectrometric analysis Homo sapiens

Synonyms

Synonyms Comment Organism
GAPDH
-
Homo sapiens
glyceraldehyde-3-phosphate dehydrogenase
-
Homo sapiens
hGAPDH
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens sequence calculation
-
8.3

General Information

General Information Comment Organism
additional information denatured GAPDH, in contrast to the native enzyme, interacts with the bacterial chaperonin GroEL and beta-amyloid peptide 1-42 Homo sapiens
physiological function glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme, whose main role is to provide energy for different cellular functions. Also the enzyme appears to be involved in numerous cell processes that have no relation to glycolysis Homo sapiens