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Literature summary for 1.2.1.12 extracted from

  • Chetri, P.B.; Shukla, R.; Tripathi, T.
    Identification and characterization of glyceraldehyde 3-phosphate dehydrogenase from Fasciola gigantica (2019), Parasitol. Res., 118, 861-872 .
    View publication on PubMed

Application

Application Comment Organism
drug development the conformation of FgGAPDH in this region is similar to the human enzyme. Therefore, GAPDH may not be a suitable target for drug discovery against fascioliasis caused by Fasciola gigantica Fasciola gigantica

Cloned(Commentary)

Cloned (Comment) Organism
gene gapdh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha Fasciola gigantica

General Stability

General Stability Organism
higher salt (300 mM NaCl) concentrations stabilizes the tetrameric state. Also binding of the cofactor NAD+ causes a conformational rearrangement in the enzyme structure, leading to the stabilization of the enzyme Fasciola gigantica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Fasciola gigantica
0.612
-
NAD+ pH 7.4, 37°C, recombinant enzyme Fasciola gigantica
1.44
-
D-glyceraldehyde 3-phosphate pH 7.4, 37°C, recombinant enzyme Fasciola gigantica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
145000
-
recombinant His-tagged enzyme, gel filtration Fasciola gigantica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Fasciola gigantica
-
3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Fasciola gigantica A0A504YZJ3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography and dialysis, to homogeneity Fasciola gigantica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Fasciola gigantica 3-phospho-D-glyceroyl phosphate + NADH + H+
-
?
additional information substrate and cofactor binding analysis and kinetics, overview Fasciola gigantica ?
-
-

Subunits

Subunits Comment Organism
homotetramer 4 * 37000, recombinant His-tagged enzyme, SDS-PAGE Fasciola gigantica

Synonyms

Synonyms Comment Organism
FgGAPDH
-
Fasciola gigantica
GAPDH
-
Fasciola gigantica
glyceraldehyde 3-phosphate dehydrogenase
-
Fasciola gigantica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Fasciola gigantica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.43
-
NAD+ pH 7.4, 37°C, recombinant enzyme Fasciola gigantica
1.58
-
D-glyceraldehyde 3-phosphate pH 7.4, 37°C, recombinant enzyme Fasciola gigantica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Fasciola gigantica

Cofactor

Cofactor Comment Organism Structure
NAD+ binding of NAD+ cofactor causes a conformational rearrangement in the enzyme structure, leading to the stabilization of the enzyme Fasciola gigantica

pI Value

Organism Comment pI Value Maximum pI Value
Fasciola gigantica sequence calculation
-
7.1

General Information

General Information Comment Organism
metabolism glyceraldehyde 3-phosphate dehydrogenase (FgGAPDH) is a key enzyme of the glycolytic pathway Fasciola gigantica
additional information docking analysis of cofactor NAD+ and substrate glyceraldehyde 3-phosphate, identification and structure analysis of the binding sites, overview. Enzyme structure homology modeling using the structure of NAD+ bound BmGAPDH from Brugia malayi (PDB ID 4K9D) as template Fasciola gigantica
physiological function in Fasciola gigantica, the glyceraldehyde 3-phosphate dehydrogenase (FgGAPDH) is a key enzyme of the glycolytic pathway and catalyzes the reversible oxidative phosphorylation of D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate, with the simultaneous reduction of NAD+ to NADH. The enzyme has various roles in the parasite Fasciola gigantica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.097
-
D-glyceraldehyde 3-phosphate pH 7.4, 37°C, recombinant enzyme Fasciola gigantica
2.337
-
NAD+ pH 7.4, 37°C, recombinant enzyme Fasciola gigantica