Cloned (Comment) | Organism |
---|---|
gene GAPDH1, DNA and amino acid sequence determination and analysis, isozyme sequence comparisons, recombinant overexpression in Mortierella alpina using Agrobacterium tumefaciens-mediated transformation | Mortierella alpina |
gene GAPDH2, DNA and amino acid sequence determination and analysis, isozyme sequence comparisons, recombinant expression using Agrobacterium tumefaciens-mediated transformation | Mortierella alpina |
Protein Variants | Comment | Organism |
---|---|---|
additional information | GAPDH1 gene silencing using Agrobacterium tumefaciens strain AGL-1-mediated transformation o the cells with siRNA. RNA interference of isozymes GAPDH1 and GAPDH2 (MA-RGAPDH1 and MA-RGAPDH2) greatly reduced the biomass of the fungus. The lipid content of MA-RGAPDH2 is about 23% higher than that of the control. Both of the lipid-increasing transformants show a higher NADPH/NADP ratio. Analysis of metabolite and enzyme expression levels reveals that the increased lipid content of MA-GAPDH1 is due to enhanced flux of glyceraldehyde-3-phosphate to glycerate-1,3-biphosphate. MA-RGAPDH2 is found to strengthen the metabolic flux of dihydroxyacetone phosphate to glycerol-3-phosphate | Mortierella alpina |
additional information | GAPDH1 gene silencing using Agrobacterium tumefaciens strain AGL-1-mediated transformation of the cells with siRNA. RNA interference of isozymes GAPDH1 and GAPDH2 (MA-RGAPDH1 and MA-RGAPDH2) greatly reduced the biomass of the fungus. The lipid content of MA-RGAPDH2 is about 23% higher than that of the control. Both of the lipid-increasing transformants show a higher NADPH/NADP ratio. Analysis of metabolite and enzyme expression levels reveals that the increased lipid content of MA-GAPDH1 is due to enhanced flux of glyceraldehyde-3-phosphate to glycerate-1,3-biphosphate | Mortierella alpina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Mortierella alpina | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Mortierella alpina ATCC 32222 | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mortierella alpina | A0A650AW89 | - |
- |
Mortierella alpina | A0A650AW98 | - |
- |
Mortierella alpina ATCC 32222 | A0A650AW89 | - |
- |
Mortierella alpina ATCC 32222 | A0A650AW98 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Mortierella alpina | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Mortierella alpina ATCC 32222 | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GAPDH | - |
Mortierella alpina |
GAPDH1 | - |
Mortierella alpina |
GAPDH2 | - |
Mortierella alpina |
glyceraldehyde-3-phosphate dehydrogenases | - |
Mortierella alpina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mortierella alpina |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mortierella alpina |
General Information | Comment | Organism |
---|---|---|
malfunction | RNA interference of isozymes GAPDH1 and GAPDH2 (MA-RGAPDH1 and MA-RGAPDH2) greatly reduced the biomass of the fungus. The lipid content of MA-RGAPDH2 is about 23% higher than that of the control. Both of the lipid-increasing transformants show a higher NADPH/NADP ratio. Analysis of metabolite and enzyme expression levels reveals that the increased lipid content of MA-GAPDH1 is due to enhanced flux of glyceraldehyde-3-phosphate to glycerate-1,3-biphosphate. Genetic manipulation of gapdh1 and gapdh2 affects the biomass and total fatty acid of Mortierella alpina through an altered NADPH/NADP ratio | Mortierella alpina |
malfunction | RNA interference of isozymes GAPDH1 and GAPDH2 (MA-RGAPDH1 and MA-RGAPDH2) greatly reduced the biomass of the fungus. The lipid content of MA-RGAPDH2 is about 23% higher than that of the control. Both of the lipid-increasing transformants show a higher NADPH/NADP ratio. Analysis of metabolite and enzyme expression levels reveals that the increased lipid content of MA-GAPDH1 is due to enhanced flux of glyceraldehyde-3-phosphate to glycerate-1,3-biphosphate. MA-RGAPDH2 is found to strengthen the metabolic flux of dihydroxyacetone phosphate to glycerol-3-phosphate. Genetic manipulation of gapdh1 and gapdh2 affects the biomass and total fatty acid of Mortierella alpina through an altered NADPH/NADP ratio | Mortierella alpina |
metabolism | different roles of GAPDH1 and GAPDH2 in lipid biosynthesis in Mortierella alpina | Mortierella alpina |
metabolism | different roles of GAPDH1 and GAPDH2 in lipid biosynthesis in Mortierella alpina. The GAPDH2 might be a moonlighting protein | Mortierella alpina |
physiological function | isozyme GAPDH1 contributes to NADPH supply and lipid accumulation in Mortierella alpina, and has a distinct role from isozyme GAPDH2. Transcriptional analysis of genes gapdh1 and gapdh2 shows that they have opposing roles during lipid accumulation. GAPDH1 possesses a stronger catalyzing ability than GAPDH2 | Mortierella alpina |