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Literature summary for 1.2.1.12 extracted from

  • Ding, X.; Wang, L.; Chen, M.; Wu, Y.; Ge, S.; Li, J.; Fan, X.; Lin, M.
    Sperm-specific glycolysis enzyme glyceraldehyde-3-phosphate dehydrogenase regulated by transcription factor SOX10 to promote uveal melanoma tumorigenesis (2021), Front. Cell Dev. Biol., 9, 610683 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine GAPDHS, which is regulated by SOX10, controls glycolysis and contributes to uveal melanoma tumorigenesis, is a therapeutic target in cancer therapy Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene gapA, quantitative real-time PCR enzyme expression analysis, GAPDHS overexpression in melanoma cells (OCM1 cells), phenotype with and without SOX10 knockout, overview Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information knockdown of GAPDHS in uveal melanoma (UM) cell lines hinders glycolysis by decreasing glucose uptake, lactate production, ATP generation, cell growth and proliferation. Conversely, overexpression of GAPDHS promotes glycolysis, cell growth and proliferation. Transcription factor SOX10 knockdown reduces the activation of GAPDHS, leading to an attenuated malignant phenotype, and SOX10 overexpression promotes the activation of GAPDHS, leading to an enhanced malignant phenotype Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Homo sapiens
-
3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O14556
-
-

Source Tissue

Source Tissue Comment Organism Textmining
eye
-
Homo sapiens
-
additional information sperm-specific enzyme GAPDHS is highly expressed in melanoma cells Homo sapiens
-
MUM-2B cell
-
Homo sapiens
-
OCM-1 cell
-
Homo sapiens
-
OM-431 cell
-
Homo sapiens
-
semen
-
Homo sapiens
-
uveal melanoma cell GAPDHS displays significantly higher expression in uveal melanoma (UM) than in normal controls Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Homo sapiens 3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
GAPDHS
-
Homo sapiens
glyceraldehyde-3-phosphate dehydrogenase
-
Homo sapiens
sperm-specific glyceraldehyde-3-phosphate dehydrogenase
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens

Expression

Organism Comment Expression
Homo sapiens SOX10 modulates GAPDHS expression by binding to the promoter. Transcription factor SOX10 knockdown reduces the activation of GAPDHS, leading to an attenuated malignant phenotype, and SOX10 overexpression promotes the activation of GAPDHS, leading to an enhanced malignant phenotype up

General Information

General Information Comment Organism
malfunction knockdown of GAPDHS in uveal melanoma (UM) cell lines hinders glycolysis by decreasing glucose uptake, lactate production, ATP generation, cell growth and proliferation. Conversely, overexpression of GAPDHS promotes glycolysis, cell growth and proliferation. Transcription factor SOX10 knockdown reduces the activation of GAPDHS, leading to an attenuated malignant phenotype, and SOX10 overexpression promotes the activation of GAPDHS, leading to an enhanced malignant phenotype. Mechanistically, SOX10 exerts its function by binding to the promoter of GAPDHS to regulate its expression. Importantly, SOX10 abrogation suppresses in vivo tumor growth and proliferation Homo sapiens
metabolism enzyme GAPDHS is essential in glycolysis Homo sapiens
physiological function sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDHS) switches glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate by coupling with the reduction of NAD+ to NADH. The sperm-specific glycolysis enzyme is regulated by transcription factor SOX10 to promote uveal melanoma (UM) tumorigenesis. GAPDHS, which is regulated by SOX10, controls glycolysis and contributes to UM tumorigenesis. GAPDHS is involved in regulating the Warburg effect in UM cells. GAPDHS serves as a functional target gene in SOX10-mediated tumor proliferation and glycolysis in UM Homo sapiens