Application | Comment | Organism |
---|---|---|
medicine | GAPDHS, which is regulated by SOX10, controls glycolysis and contributes to uveal melanoma tumorigenesis, is a therapeutic target in cancer therapy | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene gapA, quantitative real-time PCR enzyme expression analysis, GAPDHS overexpression in melanoma cells (OCM1 cells), phenotype with and without SOX10 knockout, overview | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | knockdown of GAPDHS in uveal melanoma (UM) cell lines hinders glycolysis by decreasing glucose uptake, lactate production, ATP generation, cell growth and proliferation. Conversely, overexpression of GAPDHS promotes glycolysis, cell growth and proliferation. Transcription factor SOX10 knockdown reduces the activation of GAPDHS, leading to an attenuated malignant phenotype, and SOX10 overexpression promotes the activation of GAPDHS, leading to an enhanced malignant phenotype | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Homo sapiens | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O14556 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
eye | - |
Homo sapiens | - |
additional information | sperm-specific enzyme GAPDHS is highly expressed in melanoma cells | Homo sapiens | - |
MUM-2B cell | - |
Homo sapiens | - |
OCM-1 cell | - |
Homo sapiens | - |
OM-431 cell | - |
Homo sapiens | - |
semen | - |
Homo sapiens | - |
uveal melanoma cell | GAPDHS displays significantly higher expression in uveal melanoma (UM) than in normal controls | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Homo sapiens | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GAPDHS | - |
Homo sapiens |
glyceraldehyde-3-phosphate dehydrogenase | - |
Homo sapiens |
sperm-specific glyceraldehyde-3-phosphate dehydrogenase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | SOX10 modulates GAPDHS expression by binding to the promoter. Transcription factor SOX10 knockdown reduces the activation of GAPDHS, leading to an attenuated malignant phenotype, and SOX10 overexpression promotes the activation of GAPDHS, leading to an enhanced malignant phenotype | up |
General Information | Comment | Organism |
---|---|---|
malfunction | knockdown of GAPDHS in uveal melanoma (UM) cell lines hinders glycolysis by decreasing glucose uptake, lactate production, ATP generation, cell growth and proliferation. Conversely, overexpression of GAPDHS promotes glycolysis, cell growth and proliferation. Transcription factor SOX10 knockdown reduces the activation of GAPDHS, leading to an attenuated malignant phenotype, and SOX10 overexpression promotes the activation of GAPDHS, leading to an enhanced malignant phenotype. Mechanistically, SOX10 exerts its function by binding to the promoter of GAPDHS to regulate its expression. Importantly, SOX10 abrogation suppresses in vivo tumor growth and proliferation | Homo sapiens |
metabolism | enzyme GAPDHS is essential in glycolysis | Homo sapiens |
physiological function | sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDHS) switches glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate by coupling with the reduction of NAD+ to NADH. The sperm-specific glycolysis enzyme is regulated by transcription factor SOX10 to promote uveal melanoma (UM) tumorigenesis. GAPDHS, which is regulated by SOX10, controls glycolysis and contributes to UM tumorigenesis. GAPDHS is involved in regulating the Warburg effect in UM cells. GAPDHS serves as a functional target gene in SOX10-mediated tumor proliferation and glycolysis in UM | Homo sapiens |