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Literature summary for 1.2.1.12 extracted from

  • Moreno, J.C.; Rojas, B.E.; Vicente, R.; Gorka, M.; Matz, T.; Chodasiewicz, M.; Peralta-Ariza, J.S.; Zhang, Y.; Alseekh, S.; Childs, D.; Luzarowski, M.; Nikoloski, Z.; Zarivach, R.; Walther, D.; Hartman, M.D.; Figueroa, C.M.; Iglesias, A.A.; Fernie, A.R.; Skirycz, A.
    Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolism (2021), EMBO J., 40, e106800 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.12

Protein Variants

Protein Variants Comment Organism
additional information generation of a gapc1/gapc2 double mutant that is entirely devoid of the cytosolic GAPC activity and insensitive to Tyr-Asp inhibition of GAPC activity Arabidopsis thaliana
additional information generation of a gapc1/gapc2 double mutant that is entirely devoid of the cytosolic GAPC activity and insensitive to Tyr-Asp inhibition of GAPC activity Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by treatment with single amino acids (Tyr and Asp) or chemically unrelated dipeptide (Ile-Glu); no inhibition by treatment with single amino acids (Tyr and Asp) or chemically unrelated dipeptide (Ile-Glu) Arabidopsis thaliana
additional information no inhibition by treatment with single amino acids (Tyr and Asp) or chemically unrelated dipeptide (Ile-Glu); no inhibition by treatment with single amino acids (Tyr and Asp) or chemically unrelated dipeptide (Ile-Glu) Nicotiana tabacum
Tyr-Asp a proteogenic dipeptide Tyr-Asp acting as regulatory small molecule, which improves plant tolerance to oxidative stress by directly interfering with glucose metabolism. Tyr-Asp feeding induced a shift of glucose 6-phosphate (G6P) utilization from glycolysis to the pentose phosphate pathway (PPP), thereby altering redox equilibrium of the NADP(H) pool and improving tolerance to oxidative stress. 23% inhibition at 0.1 mM. Tyr-Asp treatment improves plant performance under stress conditions; a proteogenic dipeptide Tyr-Asp acting as regulatory small molecule, which improves plant tolerance to oxidative stress by directly interfering with glucose metabolism. Tyr-Asp feeding induced a shift of glucose 6-phosphate (G6P) utilization from glycolysis to the pentose phosphate pathway (PPP), thereby altering redox equilibrium of the NADP(H) pool and improving tolerance to oxidative stress. 23% inhibition at 0.1 mM. Tyr-Asp treatment improves plant performance under stress conditions Arabidopsis thaliana
Tyr-Asp a proteogenic dipeptide Tyr-Asp acting as regulatory small molecule, which improves plant tolerance to oxidative stress by directly interfering with glucose metabolism. Tyr-Asp feeding induced a shift of glucose 6-phosphate (G6P) utilization from glycolysis to the pentose phosphate pathway (PPP), thereby altering redox equilibrium of the NADP(H) pool and improving tolerance to oxidative stress. 23% inhibition at 0.1 mM. Tyr-Asp treatment improves plant performance under stress conditions; a proteogenic dipeptide Tyr-Asp acting as regulatory small molecule, which improves plant tolerance to oxidative stress by directly interfering with glucose metabolism. Tyr-Asp feeding induced a shift of glucose 6-phosphate (G6P) utilization from glycolysis to the pentose phosphate pathway (PPP), thereby altering redox equilibrium of the NADP(H) pool and improving tolerance to oxidative stress. 23% inhibition at 0.1 mM. Tyr-Asp treatment improves plant performance under stress conditions Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Arabidopsis thaliana 5829
-
cytosol
-
 Nicotiana tabacum 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Arabidopsis thaliana
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Nicotiana tabacum
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P25858
-
-
Arabidopsis thaliana Q9FX54
-
-
Nicotiana tabacum A0A0K2GN10
-
-
Nicotiana tabacum A0A0K2GP10
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Arabidopsis thaliana
-
seedling
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Arabidopsis thaliana 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Nicotiana tabacum 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
GAPC
-
 Arabidopsis thaliana
GAPC
-
 Nicotiana tabacum
GapC1
-
 Arabidopsis thaliana
GapC1
-
 Nicotiana tabacum
GapC2
-
 Arabidopsis thaliana
GapC2
-
 Nicotiana tabacum
glyceraldehyde 3-phosphate dehydrogenase
-
 Arabidopsis thaliana
glyceraldehyde 3-phosphate dehydrogenase
-
 Nicotiana tabacum
phosphorylating NAD+-dependent GAPDH
-
 Arabidopsis thaliana
phosphorylating NAD+-dependent GAPDH
-
 Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Arabidopsis thaliana
NAD+
-
 Nicotiana tabacum

General Information

General Information Comment Organism
malfunction Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolism. Tyr-Asp inhibits the activity of a key glycolytic enzyme, glyceraldehyde 3-phosphate dehydrogenase (GAPC), and redirects glucose toward pentose phosphate pathway (PPP) and NADPH production. Tyr-Asp supplementation improves the growth performance of both Arabidopsis and tobacco seedlings subjected to oxidative stress conditions. Neither the combination of Tyr and Asp nor the two other tested dipeptides, Ser-Leu and Gly-Pro, exhibit the bioactivity of Tyr-Asp. Tyr-Asp treatment, but neither the combination of amino acids nor the two other tested dipeptides improves plant performance under stress conditions. Tyr-Asp supplementation increases biomass of catechin-treated wild-type seedlings. The Tyr-Asp-associated stress tolerance is dependent on the inhibition of the GAPC1 and GAPC2 activities. Proteome characterization of the Tyr-Asp feeding experiment revealed changes in protein and redox metabolism consistent with the Tyr-Asp protein interactions beyond that with GAPC, Tyr-Asp affects redox and protein metabolism, phenotypes, overview Arabidopsis thaliana
malfunction Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolism. Tyr-Asp inhibits the activity of a key glycolytic enzyme, glyceraldehyde 3-phosphate dehydrogenase (GAPC), and redirects glucose toward pentose phosphate pathway (PPP) and NADPH production. Tyr-Asp supplementation improves the growth performance of both Arabidopsis and tobacco seedlings subjected to oxidative stress conditions. Neither the combination of Tyr and Asp nor the two other tested dipeptides, Ser-Leu and Gly-Pro, exhibit the bioactivity of Tyr-Asp. Tyr-Asp treatment, but neither the combination of amino acids nor the two other tested dipeptides improves plant performance under stress conditions. Tyr-Asp supplementation increases biomass of catechin-treated wild-type seedlings. The Tyr-Asp-associated stress tolerance is dependent on the inhibition of the GAPC1 and GAPC2 activities. Proteome characterization of the Tyr-Asp feeding experiment revealed changes in protein and redox metabolism consistent with the Tyr-Asp protein interactions beyond that with GAPC, Tyr-Asp affects redox and protein metabolism, phenotypes, overview Nicotiana tabacum
metabolism in the cytosol, two different GAPDHs are involved in glycolysis, the phosphorylating NAD+-dependent GAPDH (GAPC1 and GAPC2, EC 1.2.1.12) and the non-phosphorylating, NADP+-dependent GAPDH (GAPN, EC 1.2.1.9). GAPN irreversibly oxidizes G3P to 3-phosphoglycerate (3PGA) and has no homology to GAPC. Besides their role in carbon assimilation and partitioning, phosphorylating GAPDHs (particularly, GAPC1 and GAPA1) have additional moonlighting functionalities Nicotiana tabacum
metabolism in the cytosol, two different GAPDHs are involved in glycolysis, the phosphorylating NAD+-dependent GAPDH (GAPC1 and GAPC2; EC 1.2.1.12) and the non-phosphorylating, NADP+-dependent GAPDH (GAPN, EC 1.2.1.9). GAPN irreversibly oxidizes G3P to 3-phosphoglycerate (3PGA) and has no homology to GAPC. Besides their role in carbon assimilation and partitioning, phosphorylating GAPDHs (particularly, GAPC1 and GAPA1) have additional moonlighting functionalities Arabidopsis thaliana
metabolism in the cytosol, two different GAPDHs are involved in glycolysis, the phosphorylating NAD+-dependent GAPDH (GAPC1 and GAPC2; EC 1.2.1.12) and the non-phosphorylating, NADP+-dependent GAPDH (GAPN, EC 1.2.1.9). GAPN irreversibly oxidizes G3P to 3-phosphoglycerate (3PGA) and has no homology to GAPC. Besides their role in carbon assimilation and partitioning, phosphorylating GAPDHs (particularly, GAPC1 and GAPA1) have additional moonlighting functionalities Nicotiana tabacum
additional information proteogenic dipeptides act as evolutionarily conserved small-molecule regulators at the nexus of stress, protein degradation, and metabolism Arabidopsis thaliana
additional information proteogenic dipeptides act as evolutionarily conserved small-molecule regulators at the nexus of stress, protein degradation, and metabolism Nicotiana tabacum