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Literature summary for 1.2.1.12 extracted from

  • Anoman, A.D.; Munoz-Bertomeu, J.; Rosa-Tellez, S.; Flores-Tornero, M.; Serrano, R.; Bueso, E.; Fernie, A.R.; Segura, J.; Ros, R.
    Plastidial glycolytic glyceraldehyde-3-phosphate dehydrogenase is an important determinant in the carbon and nitrogen metabolism of heterotrophic cells in Arabidopsis (2015), Plant Physiol., 169, 1619-1637 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of enzyme gapcp double mutants, gapcp1gapcp2, under the control of photosynthetic (Rubisco small subunit RBCS2B [RBCS]) or heterotrophic (phosphate transporter PHT1.2 [PHT]) cell-specific promoters. Expression of GAPCp1 under the control of RBCS in gapcp1gapcp2 has no significant effect on the metabolite profile or growth in the aerial part (AP). GAPCp1 expression under the control of the PHT promoter clearly affects Arabidopsis thaliana development by increasing the number of lateral roots and having a major effect on AP growth and metabolite profile. Recombinant expression of GFP-tagged isozyme GAPCp1 in Arabidopsis thaliana, quantitative real-time-PCR expression analysis Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of enzyme gapcp double mutants, gapcp1gapcp2, under the control of photosynthetic (Rubisco small subunit RBCS2B [RBCS]) or heterotrophic (phosphate transporter PHT1.2 [PHT]) cell-specific promoters Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-
chloroplast plastidial glycolytic isoform 2 of glyceraldehyde-3-phosphate dehydrogenase, GAPCp2 Arabidopsis thaliana 9507
-
plastid plastidial glycolytic isoform 1 of glyceraldehyde-3-phosphate dehydrogenase, GAPCp1 Arabidopsis thaliana 9536
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Arabidopsis thaliana
-
3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q5E924
-
-
Arabidopsis thaliana Q9SAJ6
-
-

Source Tissue

Source Tissue Comment Organism Textmining
aerial part
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
meristem
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Arabidopsis thaliana 3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
GAPCp1
-
Arabidopsis thaliana
GAPCp2
-
Arabidopsis thaliana
glyceraldehyde-3-phosphate dehydrogenase
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction transcriptomic and metabolomic analyses indicate that the lack of GAPCp activity affects nitrogen and carbon metabolism as well as mineral nutrition and that glycerate and glutamine are the main metabolites responding to GAPCp activity, phenotypic analysis, detailed overview. Mutants gapcp1gapcp2 display a drastic reduction not only of root growth but also of the aerial part (AP) when grown both on plates and in greenhouse conditions. This phenotype is observed in double homozygous mutants only. Single mutants (gapcp1 or gapcp2) or mutant plants homozygous for one of the genes and heterozygous for the other are phenotypically indistinguishable from the wild-type plants. At the adult stage, GAPCp1 expression in the AP is able to complement the sterile phenotype of gapcp1gapcp2, resulting in plants with siliques and fertile seeds. The developmental pattern of gapcp1gapcp2 RBCS:GAPCp1 is also altered as compared with gapcp1gapcp2, probably as a consequence of the fertile phenotype, displaying shorter shoots than gapcp1gapcp2. A similar developmental pattern alteration is observed in the sterile gapcp1gapcp2 35S:GAPCp1 lines Arabidopsis thaliana
malfunction transcriptomic and metabolomic analyses indicate that the lack of GAPCp activity affects nitrogen and carbon metabolism as well as mineral nutrition and that glycerate and glutamine are the main metabolites responding to GAPCp activity, phenotypic analysis, detailed overview. Mutants gapcp1gapcp2 display a drastic reduction not only of root growth but also of the aerial part when grown both on plates and in greenhouse conditions. This phenotype is observed in double homozygous mutants only. Single mutants (gapcp1 or gapcp2) or mutant plants homozygous for one of the genes and heterozygous for the other are phenotypically indistinguishable from the wild-type plants Arabidopsis thaliana
metabolism GAPCp might be an important metabolic connector of glycolysis with other pathways, such as the phosphorylated pathway of serine biosynthesis, the ammonium assimilation pathway, or the metabolism of gamma-aminobutyrate, which in turn affect plant development Arabidopsis thaliana
metabolism plastidial isozyme GAPCp might be an important metabolic connector of glycolysis with other pathways, such as the phosphorylated pathway of serine biosynthesis, the ammonium assimilation pathway, or the metabolism of gamma-aminobutyrate, which in turn affect plant development Arabidopsis thaliana
physiological function plastidial isozymes GAPCps are critical for primary root growth and essential for microspore development. Plastidial isozyme GAPCp1 is not functionally important in photosynthetic cells but plays a fundamental role in roots and in heterotrophic cells of the aerial part. GAPCp1 expression in reproductive organs is necessary for Arabidopsis fertility. GAPCp activity may be required in root meristems and the root cap for normal primary root growth. GAPCp might be an important metabolic connector of glycolysis with other pathways, such as the phosphorylated pathway of serine biosynthesis, the ammonium assimilation pathway, or the metabolism of gamma-aminobutyrate, which in turn affect plant development. Isozymes GAPCp1 and GAPCp2 are redundant to one another Arabidopsis thaliana
physiological function the plastidial GAPCps are critical for primary root growth and essential for microspore development. Plastidial isozyme GAPCp might be an important metabolic connector of glycolysis with other pathways, such as the phosphorylated pathway of serine biosynthesis, the ammonium assimilation pathway, or the metabolism of gamma-aminobutyrate, which in turn affect plant development. Isozymes GAPCp1 and GAPCp2 are redundant to one another Arabidopsis thaliana