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Literature summary for 1.2.1.12 extracted from

  • Kunjithapatham, R.; Geschwind, J.F.; Devine, L.; Boronina, T.N.; OMeally, R.N.; Cole, R.N.; Torbenson, M.S.; Ganapathy-Kanniappan, S.
    Occurrence of a multimeric high-molecular-weight glyceraldehyde-3-phosphate dehydrogenase in human serum (2015), J. Proteome Res., 14, 1645-1656 .
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular extracellular GAPDH in human serum is a multimeric, high-molecular-weight, yet glycolytically active enzyme. The enzyme is secreted Homo sapiens
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-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Homo sapiens
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3-phospho-D-glyceroyl phosphate + NADH + H+
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?

Organism

Organism UniProt Comment Textmining
Homo sapiens P04406
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-

Purification (Commentary)

Purification (Comment) Organism
extracellular serum isozyme by immunoaffinity purification combined with anion exchange chromatography by using DEAE affigel blue chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
breast cancer cell
-
Homo sapiens
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colon carcinoma cell
-
Homo sapiens
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HEP-3B cell
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Homo sapiens
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Hep-G2 cell
-
Homo sapiens
-
MCF-7 cell
-
Homo sapiens
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serum a multimeric high-molecular-weight glyceraldehyde-3-phosphate dehydrogenase, the isozyme is secreted. Low levels of non-serum-specific isozyme GAPDH. The serum-specific high-molecular weight isozyme is also secreted from cancer cells Homo sapiens
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SK-HEP-1 cell
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Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Homo sapiens 3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Subunits

Subunits Comment Organism
More high-molecular-weight multimers of serum GAPDH, multiple protein bands (about 25, and 50-75 kDa) along with some proteins with weak signals in albumin-depleted serum samples, SDS-PAGE and mass spectrometry. The enzymatic function of serum GAPDH remained unaffected by the multimers Homo sapiens

Synonyms

Synonyms Comment Organism
GAPDH
-
Homo sapiens
glyceraldehyde-3-phosphate dehydrogenase
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Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens

General Information

General Information Comment Organism
evolution cellular glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a phylogenetically conserved, ubiquitous enzyme Homo sapiens
physiological function cellular glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a phylogenetically conserved, ubiquitous enzyme that plays an indispensable role in energy metabolism. The extracellular GAPDH in human serum is a multimeric, high-molecular-weight, yet glycolytically active enzyme, the enzymatic function of serum GAPDH remained unaffected by the multimers Homo sapiens