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Literature summary for 1.2.1.12 extracted from

  • Matsumura, H.; Kai, A.; Maeda, T.; Tamoi, M.; Satoh, A.; Tamura, H.; Hirose, M.; Ogawa, T.; Kizu, N.; Wadano, A.; Inoue, T.; Shigeoka, S.
    Structure basis for the regulation of glyceraldehyde-3-phosphate dehydrogenase activity via the intrinsically disordered protein CP12 (2011), Structure, 19, 1846-1854.
    View publication on PubMed
Search for more literature for 1.2.1.12

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization of the ternary complex between GAPDH, intrinsically disordered protein CP12 and NAD+ in both the absence and presence of copper. The oxidized CP12 proten becomes partially structured upon GAPDH binding. The C-terminus of CP12 is inserted into the active-site region of GAPDH, resulting in competitive inhibition of GAPDH Synechococcus elongatus
in complex with CP12 and NAD+, hanging drop vapor diffusion method, using 20% (w/v) PEG3350 and 0.2 M magnesium acetate Synechococcus elongatus

Inhibitors

Inhibitors Comment Organism Structure
additional information the C-terminus of CP12 is inserted into the active-site region of glyceraldehyde-3-phosphate dehydrogenase, resulting in competitive inhibition of the enzyme Synechococcus elongatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36830
-
 4 * 36830, MALDI-TOF mass spectrometry Synechococcus elongatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Synechococcus elongatus
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Synechococcus elongatus PCC 7942
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Synechococcus elongatus Q9R6W2
-
-
Synechococcus elongatus PCC 7942 Q9R6W2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Synechococcus elongatus 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Synechococcus elongatus PCC 7942 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 36830, MALDI-TOF mass spectrometry Synechococcus elongatus

Synonyms

Synonyms Comment Organism
gap2
-
 Synechococcus elongatus
GAPDH
-
 Synechococcus elongatus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Synechococcus elongatus