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Literature summary for 1.2.1.12 extracted from

  • Cook, W.; Senkovich, O.; Chattopadhyay, D.
    An unexpected phosphate binding site in glyceraldehyde 3-phosphate dehydrogenase: Crystal structures of apo, holo and ternary complex of Cryptosporidium parvum enzyme (2009), BMC Struct. Biol., 9, 9-9.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Cryptosporidium parvum

Crystallization (Commentary)

Crystallization (Comment) Organism
GAPDH in the apo and holo (enzyme in complex with NAD+) state, hanging drop vapor diffusion method, using 16-20% (w/v) PEG 3000 and 0.2 M NaCl in 0.1 M HEPES, pH 7.5 Cryptosporidium parvum

Protein Variants

Protein Variants Comment Organism
C153S the specific activity of the mutant enzyme is approximately 450fold lower than that of the wild type enzyme Cryptosporidium parvum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000032
-
NAD+ wild type enzyme, in 50 mM triethanolamine, 50 mM Na2HPO4, 0.2 mM EDTA, pH 8.8, temperature not specified in the publication Cryptosporidium parvum
0.763
-
D-glyceraldehyde 3-phosphate wild type enzyme, in 50 mM triethanolamine, 50 mM Na2HPO4, 0.2 mM EDTA, pH 8.8, temperature not specified in the publication Cryptosporidium parvum

Organism

Organism UniProt Comment Textmining
Cryptosporidium parvum Q7YYQ9
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, SP Sepharose column chromatography, and Superdex 200 gel filtration Cryptosporidium parvum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Cryptosporidium parvum 3-phospho-D-glyceroyl phosphate + NADH
-
?

Subunits

Subunits Comment Organism
homotetramer x-ray crystallography Cryptosporidium parvum

Synonyms

Synonyms Comment Organism
GAPDH
-
Cryptosporidium parvum
glyceraldehyde 3-phosphate dehydrogenase
-
Cryptosporidium parvum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Cryptosporidium parvum