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Literature summary for 1.2.1.12 extracted from

  • Fourrat, L.; Iddar, A.; Soukri, A.
    Purification and characterization of cytosolic glyceraldehyde-3-phosphate dehydrogenase from the dromedary camel (2007), Acta Biochim. Biophys. Sin. (Shanghai), 39, 148-154.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ strong inhibition Camelus dromedarius
Cu2+
-
Camelus dromedarius
Hg2+ strong inhibition Camelus dromedarius
K+
-
Camelus dromedarius
Mn2+
-
Camelus dromedarius
Zn2+
-
Camelus dromedarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
NAD+ in 50 mM tricine-NaOH buffer (pH 8.5) with 10 mM sodium arsenate, at 25°C Camelus dromedarius
0.21
-
D-glyceraldehyde 3-phosphate in 50 mM tricine-NaOH buffer (pH 8.5) with 10 mM sodium arsenate, at 25°C Camelus dromedarius

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Camelus dromedarius 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36500
-
4 * 36500, non-denaturing PAGE Camelus dromedarius
37000
-
SDS-PAGE Camelus dromedarius
146000
-
non-denaturing PAGE Camelus dromedarius

Organism

Organism UniProt Comment Textmining
Camelus dromedarius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation and Blue Sepharose column chromatography Camelus dromedarius

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
Camelus dromedarius
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.68
-
crude extract, at 25°C Camelus dromedarius
45.1
-
after 26.8fold purification, at 25°C Camelus dromedarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Camelus dromedarius 3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 37000, SDS-PAGE Camelus dromedarius
homotetramer 4 * 36500, non-denaturing PAGE Camelus dromedarius

Synonyms

Synonyms Comment Organism
GAPDH
-
Camelus dromedarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28 32
-
Camelus dromedarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15 70 pre-incubation for 10 min at temperatures varying between 20 and 32°C does not irreversibly affect the enzyme activity, thermal inactivation occurs above 35°C and results in total activity loss at 70°C Camelus dromedarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Camelus dromedarius

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5
-
Camelus dromedarius

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent Camelus dromedarius

pI Value

Organism Comment pI Value Maximum pI Value
Camelus dromedarius isoelectric focusing
-
7.2

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.6
-
at 25°C Camelus dromedarius Cd2+
6.2
-
at 25°C Camelus dromedarius Hg2+
14.2
-
at 25°C Camelus dromedarius Zn2+
22.7
-
at 25°C Camelus dromedarius Cu2+
25.8
-
at 25°C Camelus dromedarius Mn2+
34.6
-
at 25°C Camelus dromedarius K+