Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | strong inhibition | Camelus dromedarius | |
Cu2+ | - |
Camelus dromedarius | |
Hg2+ | strong inhibition | Camelus dromedarius | |
K+ | - |
Camelus dromedarius | |
Mn2+ | - |
Camelus dromedarius | |
Zn2+ | - |
Camelus dromedarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
NAD+ | in 50 mM tricine-NaOH buffer (pH 8.5) with 10 mM sodium arsenate, at 25°C | Camelus dromedarius | |
0.21 | - |
D-glyceraldehyde 3-phosphate | in 50 mM tricine-NaOH buffer (pH 8.5) with 10 mM sodium arsenate, at 25°C | Camelus dromedarius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Camelus dromedarius | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36500 | - |
4 * 36500, non-denaturing PAGE | Camelus dromedarius |
37000 | - |
SDS-PAGE | Camelus dromedarius |
146000 | - |
non-denaturing PAGE | Camelus dromedarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camelus dromedarius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation and Blue Sepharose column chromatography | Camelus dromedarius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Camelus dromedarius | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.68 | - |
crude extract, at 25°C | Camelus dromedarius |
45.1 | - |
after 26.8fold purification, at 25°C | Camelus dromedarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Camelus dromedarius | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 37000, SDS-PAGE | Camelus dromedarius |
homotetramer | 4 * 36500, non-denaturing PAGE | Camelus dromedarius |
Synonyms | Comment | Organism |
---|---|---|
GAPDH | - |
Camelus dromedarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | 32 | - |
Camelus dromedarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 70 | pre-incubation for 10 min at temperatures varying between 20 and 32°C does not irreversibly affect the enzyme activity, thermal inactivation occurs above 35°C and results in total activity loss at 70°C | Camelus dromedarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Camelus dromedarius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Camelus dromedarius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent | Camelus dromedarius |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Camelus dromedarius | isoelectric focusing | - |
7.2 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
4.6 | - |
at 25°C | Camelus dromedarius | Cd2+ | |
6.2 | - |
at 25°C | Camelus dromedarius | Hg2+ | |
14.2 | - |
at 25°C | Camelus dromedarius | Zn2+ | |
22.7 | - |
at 25°C | Camelus dromedarius | Cu2+ | |
25.8 | - |
at 25°C | Camelus dromedarius | Mn2+ | |
34.6 | - |
at 25°C | Camelus dromedarius | K+ |