Application | Comment | Organism |
---|---|---|
medicine | potential antimalarial target | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
into the pET15b vector for expression in Escherichia coli BL21DE3 cells | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
crystals of tag-free protein diffracts X-rays to 2.6 A resolution, of the His-tagged protein to 4.0 A | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ferriprotoporphyrin IX | - |
Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
monomer | Plasmodium falciparum |
130000 | - |
tetramer, estimated by gel filtration | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Plasmodium falciparum | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the filtered supernatant of the centrifuged cell lysate is applied onto 3 ml Talon resin, the His-tag is removed, further purification by gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Plasmodium falciparum | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
GAPDH | - |
Plasmodium falciparum |
glyceraldehyde-3-phosphate dehydrogenase | - |
Plasmodium falciparum |
PfGAPDH | - |
Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | one molecule NAD+ is bound to each of the four subunits of the tetrameric enzyme | Plasmodium falciparum |