Crystallization (Comment) | Organism |
---|---|
after addition of NAD+ to the apoprotein | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | inhibition of esterase activity with p-nitrophenyl acetate | Escherichia coli | |
AMP | inhibition of esterase activity with p-nitrophenyl acetate | Escherichia coli | |
ATP | inhibition of esterase activity with p-nitrophenyl acetate | Escherichia coli | |
iodoacetate | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, high speed equilibrium sedimentation after treatment with 5 M guanidine hydrochloride containing 0.01 M dithiothreitol | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Escherichia coli | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
p-nitrophenyl acetate + H2O | - |
Escherichia coli | p-nitrophenol + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, high speed equilibrium sedimentation after treatment with 5 M guanidine hydrochloride containing 0.01 M dithiothreitol | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
50 mM Tris-chloride buffer and triethanolamine-chloride buffer | Escherichia coli |
8.8 | - |
50 mM sodium diphosphate buffer | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | about 85% of maximal activity at pH 7.5 and pH 8.5, 50 mM Tris-chloride buffer. About 75% of maximal activity at pH 7.5 and pH 8.5, 50 mM triethanolamine-chloride buffer | Escherichia coli |
8.3 | 9.3 | 85% of maximal activity at pH 8.3 and pH 9.3, 50 mM sodium diphosphate buffer | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | contains less than 0.3 mol of NAD+ per mol of native protein | Escherichia coli |