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Literature summary for 1.2.1.12 extracted from
- Glyceraldehyde-3-phosphate dehydrogenases from Euglena gracilis. Purification and physical and chemical characterization (1975), Arch. Biochem. Biophys., 171, 444-458.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | 10 mM, 50% inhibition | Euglena gracilis |  |
| cAMP | - |
Euglena gracilis | |
| iodoacetate | - |
Euglena gracilis | |
| NEM | 1 mM, complete inhibition | Euglena gracilis | |
| PCMB | 1 mM, complete inhibition, partially reversed by dithiothreitol; 1 mM, inhibition is partially reversed by dithiothreitol | Euglena gracilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0066 | - |
NAD+ | - |
Euglena gracilis | |
| 0.021 | - |
NADH | - |
Euglena gracilis | |
| 0.081 | - |
D-glyceraldehyde 3-phosphate | - |
Euglena gracilis | |
| 0.47 | - |
3-phospho-D-glyceroyl phosphate | - |
Euglena gracilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
4 * 37000, SDS-PAGE | Euglena gracilis |
| 137000 | - |
gel filtration | Euglena gracilis |
| 138600 | - |
sucrose density gradient centrifugation | Euglena gracilis |
| 140500 | - |
equilibrium sedimentation | Euglena gracilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euglena gracilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Euglena gracilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Euglena gracilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phospho-D-glyceroyl phosphate + NADH | - |
Euglena gracilis | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
r | |
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Euglena gracilis | 3-phospho-D-glyceroyl phosphate + NADH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 37000, SDS-PAGE | Euglena gracilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Euglena gracilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | 8.9 | - |
Euglena gracilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Euglena gracilis | |
| NADH | cofactor | Euglena gracilis | |
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