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Literature summary for 1.2.1.12 extracted from
- Glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus (1975), Methods Enzymol., 41, 268-273.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
General Stability
| General Stability | Organism |
|---|---|
| 5.0 M guanidine-HCl, rapid and irreversible inactivation at 30°C | Geobacillus stearothermophilus |
| 8.0 M urea, unusually resistant at 30°C, 40°C and 50°C. Rapid inactivation at 55°C and at 60°C | Geobacillus stearothermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Geobacillus stearothermophilus |  |
| p-hydroxymercuribenzoate | - |
Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
4 * 36000, SDS-PAGE | Geobacillus stearothermophilus |
| 148700 | - |
equilibrium sedimentation | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Geobacillus stearothermophilus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, in 67% saturated ammonium sulfate solution containing EDTA and dithiothreitol, stable for months | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + arsenate + NAD+ | - |
Geobacillus stearothermophilus | 3-phospho-D-glyceroyl arsenate + NADH | - |
ir | |
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Geobacillus stearothermophilus | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 36000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
10 min, about 10% inactivation | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Geobacillus stearothermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | enzyme contains 4 equivalents of firmly bound NAD+ | Geobacillus stearothermophilus | |
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