Any feedback?
Literature summary for 1.2.1.12 extracted from
- Glyceraldehyde-3-phosphate dehydrogenase from pea seeds (1982), Methods Enzymol., 89, 319-325.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-phospho-D-glyceroyl phosphate | - |
Pisum sativum |  |
| D-glyceraldehyde 3-phosphate | substrate inhibition | Pisum sativum | |
| NAD+ | substrate inhibition | Pisum sativum | |
| NADH | competitive with NAD+ and with D-glyceraldehyde 3-phosphate | Pisum sativum | |
| phosphate | substrate inhibition | Pisum sativum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 145000 | - |
velocity sedimentation | Pisum sativum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pisum sativum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, no substantial loss of activity after several months | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phospho-D-glyceroyl phosphate + NADH | - |
Pisum sativum | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
r | |
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Pisum sativum | 3-phospho-D-glyceroyl phosphate + NADH | - |
r | |
| D-glyceraldehyde 3-phosphate + thio-NAD+ + phosphate | thionicotinamide adenine dinucleotide is not as effective as NAD+ | Pisum sativum | 3-phospho-D-glyceroyl phosphate + thio-NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 36000-37000, SDS-PAGE | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Pisum sativum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | contains 0.5-0.7 mol of NAD+ per mol of subunit | Pisum sativum | |
| NADH | cofactor | Pisum sativum | |
| thio-NAD+ | thionicotinamide adenine dinucleotide is not as effective as NAD+ | Pisum sativum | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
