Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Koningic acid | irreversible inhibition, GAPDH I : 50% inhibition by 1 mM, no effect at 0.1 mM. GAPDH II: 50% inhibition by 0.01 mM. Under conditions of koningic acid production the koningic-acid-resistant isoenzyme GAPDH I is produced. In peptone-rich medium where non koningic acid is produced the koningic-acid-sensitive isoenzyme GAPDH II is produced in addition to GAPDH 1 | Trichoderma koningii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
NAD+ | GAPDH I | Trichoderma koningii | |
0.046 | - |
NAD+ | GAPDH II | Trichoderma koningii | |
0.2 | - |
phosphate | isoenzyme I and II | Trichoderma koningii | |
0.33 | - |
D-glyceraldehyde 3-phosphate | GAPDH II | Trichoderma koningii | |
0.54 | - |
D-glyceraldehyde 3-phosphate | GAPDH I | Trichoderma koningii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
4 * 38000, GAPDH I and GAPDH II, SDS-PAGE | Trichoderma koningii |
152000 | - |
gel filtration | Trichoderma koningii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Trichoderma koningii | GAPDH I is responsible for glycolysis when koningic acid is produced | 3-phospho-D-glyceroyl phosphate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma koningii | - |
under conditions of koningic acid production the koningic-acid-resistant isoenzyme GAPDH I is produced. In peptone-rich medium where non koningic acid is produced the koningic-acid-sensitive isoenzyme GAPDH II is produced in addition to GAPDH II | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
125 | - |
- |
Trichoderma koningii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Trichoderma koningii | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | GAPDH I is responsible for glycolysis when koningic acid is produced | Trichoderma koningii | 3-phospho-D-glyceroyl phosphate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38000, GAPDH I and GAPDH II, SDS-PAGE | Trichoderma koningii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.9 | - |
GAPDH I and GAPDH II | Trichoderma koningii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Trichoderma koningii |