Literature summary for 1.2.1.12 extracted from

Misset, O.; van Beeumen, J.; Lambeir, A.M.; van der Meer, R.; Opperdoes, F.R.
Glyceraldehyde-phosphate dehydrogenase from Trypanosoma brucei. Comparison of the glycosomal and cytosolic isoenzymes (1987), Eur. J. Biochem., 162, 501-507.

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Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Trypanosoma brucei	5829	-
glyoxysome	-	Trypanosoma brucei	9514	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33500	-	4 * 33500, cytosolic enzyme, SDS-PAGE	Trypanosoma brucei
38000	-	4 * 38000, glyoxysomal enzyme, SDS-PAGE	Trypanosoma brucei
140000	150000	sucrose density gradient centrifugation	Trypanosoma brucei

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+	-	Trypanosoma brucei	3-phospho-D-glyceroyl phosphate + NADH	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 38000, glyoxysomal enzyme, SDS-PAGE	Trypanosoma brucei
tetramer	4 * 33500, cytosolic enzyme, SDS-PAGE	Trypanosoma brucei

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	8.5	-	Trypanosoma brucei

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	9	pH 5.5: about 50% of maximal activity, pH 9.0: about 55% of maximal activity, cytosolic enzyme	Trypanosoma brucei
7	9	about 40% of maximal activity at pH 7.0 and at pH 9.0, glyoxysomal enzyme	Trypanosoma brucei

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Trypanosoma brucei

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Release 2023.1 (January 2023)