Application | Comment | Organism |
---|---|---|
medicine | target for antibiotic development | Streptococcus pneumoniae |
Cloned (Comment) | Organism |
---|---|
expression as His-tag fusion protein in Escherichia coli BL21 (DE3) | Streptococcus pneumoniae |
into a pET28a vector for expression in Escherichia coli BL21DE3 | Streptococcus pneumoniae |
Crystallization (Comment) | Organism |
---|---|
- |
Streptococcus pneumoniae |
crystallization of the apo-enzyme, in complex with NADP+, in complex with L-aspartate-beta-semialdehyde, in complex with NADP+ and L-aspartate-beta-semialdehyde | Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
NADPH | - |
Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + phosphate + NADP+ | Streptococcus pneumoniae | - |
L-4-aspartyl phosphate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity chromatography and anion exchange chromatography | Streptococcus pneumoniae |
using a Co2+ affinity matrix and anion exchange chromatography | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-4-aspartyl phosphate + NADPH | - |
Streptococcus pneumoniae | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
Streptococcus pneumoniae | L-4-aspartyl phosphate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | crystallization | Streptococcus pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
ASADH | - |
Streptococcus pneumoniae |
aspartate-beta-semialdehyde dehydrogenase | - |
Streptococcus pneumoniae |
aspartate-semialdehyde dehydrogenase | - |
Streptococcus pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
activity assay performed at room temperature | Streptococcus pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
L-aspartate-semialdehyde | - |
Streptococcus pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
activity assay | Streptococcus pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | in contrast to Gram-negative bacterial aspartate-beta-semialdehyde dehydrogenases which are specific for NADP+ the enzyme of Gram-positive showed a dual specificity for NAD+ and NADP+ | Streptococcus pneumoniae | |
NADP+ | - |
Streptococcus pneumoniae | |
NADP+ | in contrast to Gram-negative bacterial aspartate-beta-semialdehyde dehydrogenases which are specific for NADP+ the enzyme of Gram-positive showed a dual specificity for NAD+ and NADP+ | Streptococcus pneumoniae | |
NADPH | - |
Streptococcus pneumoniae |