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Literature summary for 1.2.1.11 extracted from

  • Faehnle, C.R.; Le Coq, J.; Liu, X.; Viola, R.E.
    Examination of key intermediates in the catalytic cycle of aspartate-beta-semialdehyde dehydrogenase from a gram-positive infectious bacteria (2006), J. Biol. Chem., 281, 31031-31040.
    View publication on PubMed

Application

Application Comment Organism
medicine target for antibiotic development Streptococcus pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
expression as His-tag fusion protein in Escherichia coli BL21 (DE3) Streptococcus pneumoniae
into a pET28a vector for expression in Escherichia coli BL21DE3 Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Streptococcus pneumoniae
crystallization of the apo-enzyme, in complex with NADP+, in complex with L-aspartate-beta-semialdehyde, in complex with NADP+ and L-aspartate-beta-semialdehyde Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3
-
NADPH
-
Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate 4-semialdehyde + phosphate + NADP+ Streptococcus pneumoniae
-
L-4-aspartyl phosphate + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity chromatography and anion exchange chromatography Streptococcus pneumoniae
using a Co2+ affinity matrix and anion exchange chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-4-aspartyl phosphate + NADPH
-
Streptococcus pneumoniae L-aspartate 4-semialdehyde + phosphate + NADP+
-
?
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Streptococcus pneumoniae L-4-aspartyl phosphate + NADPH + H+
-
?

Subunits

Subunits Comment Organism
homodimer crystallization Streptococcus pneumoniae

Synonyms

Synonyms Comment Organism
ASADH
-
Streptococcus pneumoniae
aspartate-beta-semialdehyde dehydrogenase
-
Streptococcus pneumoniae
aspartate-semialdehyde dehydrogenase
-
Streptococcus pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
activity assay performed at room temperature Streptococcus pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
L-aspartate-semialdehyde
-
Streptococcus pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
activity assay Streptococcus pneumoniae

Cofactor

Cofactor Comment Organism Structure
NAD+ in contrast to Gram-negative bacterial aspartate-beta-semialdehyde dehydrogenases which are specific for NADP+ the enzyme of Gram-positive showed a dual specificity for NAD+ and NADP+ Streptococcus pneumoniae
NADP+
-
Streptococcus pneumoniae
NADP+ in contrast to Gram-negative bacterial aspartate-beta-semialdehyde dehydrogenases which are specific for NADP+ the enzyme of Gram-positive showed a dual specificity for NAD+ and NADP+ Streptococcus pneumoniae
NADPH
-
Streptococcus pneumoniae