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Literature summary for 1.2.1.11 extracted from

  • Cahyanto, M.N.; Kawasaki, H.; Nagashio, M.; Fujiyama, K.; Seki, T.
    Regulation of aspartokinase, aspartate semialdehyde dehydrogenase, dihydrodipicolinate synthase and dihydrodipicolinate reductase in Lactobacillus plantarum (2006), Microbiology, 152, 105-112.
    View publication on PubMed
Search for more literature for 1.2.1.11

Application

Application Comment Organism
biotechnology development of lysine-overproducing strains Lactiplantibacillus plantarum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Hfr3000 Lactiplantibacillus plantarum
genes are amplified from chromosomal DNA of Lactobacillus plantarum and cloned into the pCR2.1-TOPO vector for sequencing, and subcloned into the pUC18 vector for heterologous expression in Escherichia coli Lactiplantibacillus plantarum

Inhibitors

Inhibitors Comment Organism Structure
petrosamine B 50% inhibition at 0.306 mM. Petrosamine B is a pyridoacridine alkaloid isolated from the sponge Oceanapia sp. Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate 4-semialdehyde + phosphate + NADP+ Lactiplantibacillus plantarum
-
 L-4-aspartyl phosphate + NADPH + H+
-
 ?
L-aspartate 4-semialdehyde + phosphate + NADP+ Lactiplantibacillus plantarum NCIMB 8826
-
 L-4-aspartyl phosphate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-
Lactiplantibacillus plantarum NCIMB 8826
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture weakly expressed Lactiplantibacillus plantarum
-
cell culture highly expressed Lactiplantibacillus plantarum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.215
-
 gene asd1 Lactiplantibacillus plantarum
2.149
-
 gene asd2 Lactiplantibacillus plantarum
2.149
-
 when expressed in Escherichia coli Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Lactiplantibacillus plantarum L-4-aspartyl phosphate + NADPH + H+
-
 ?
L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Lactiplantibacillus plantarum NCIMB 8826 L-4-aspartyl phosphate + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
ASADHD
-
 Lactiplantibacillus plantarum
Asd1
-
 Lactiplantibacillus plantarum
Asd2
-
 Lactiplantibacillus plantarum
aspartate semialdehyde dehydrogenase
-
 Lactiplantibacillus plantarum
aspartate-semialdehyde dehydrogenase
-
 Lactiplantibacillus plantarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 activity assay Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 activity assay Lactiplantibacillus plantarum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Lactiplantibacillus plantarum