Application | Comment | Organism |
---|---|---|
medicine | enzyme is a validated drug target | Mycobacterium tuberculosis |
pharmacology | inhibitor design from enzyme three-dimensional structure | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
gene asd, subcloning in Escherichia coli strain JM109, DNA sequence determination and analysis, optimization of functional overexpression in Escherichia coli strain M15 as His-tagged protein | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
NADP+ | recombinant enzyme, pH 9.0, 30°C | Mycobacterium tuberculosis | |
0.955 | - |
L-aspartate-4-semialdehyde | recombinant enzyme, pH 9.0, 30°C | Mycobacterium tuberculosis | |
11.39 | - |
phosphate | recombinant enzyme, pH 9.0, 30°C | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, about, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-4-aspartyl phosphate + NADPH | Mycobacterium tuberculosis | physiological forward reaction direction, key enzyme in diaminopimelic acid biosynthetic pathway | L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
? | |
L-4-aspartyl phosphate + NADPH | Mycobacterium tuberculosis H37Rv | physiological forward reaction direction, key enzyme in diaminopimelic acid biosynthetic pathway | L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WNX5 | strain H37 Rv, gene asd | - |
Mycobacterium tuberculosis H37Rv | P9WNX5 | strain H37 Rv, gene asd | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography to 96% purity | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
purified recombinant enzyme | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-4-aspartyl phosphate + NADPH | physiological forward reaction direction, key enzyme in diaminopimelic acid biosynthetic pathway | Mycobacterium tuberculosis | L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
? | |
L-4-aspartyl phosphate + NADPH | physiological forward reaction direction, key enzyme in diaminopimelic acid biosynthetic pathway | Mycobacterium tuberculosis H37Rv | L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
? | |
L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
Mycobacterium tuberculosis | L-4-aspartyl phosphate + NADPH | - |
r | |
L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
Mycobacterium tuberculosis H37Rv | L-4-aspartyl phosphate + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, about, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
ASD enzyme | - |
Mycobacterium tuberculosis |
aspartate-beta-semialdehyde dehydrogenase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.49 | - |
NADP+ | recombinant enzyme, pH 9.0, 30°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Mycobacterium tuberculosis | |
NADPH | - |
Mycobacterium tuberculosis |