Cloned (Comment) | Organism |
---|---|
cloning and recombinant protein overproduction with Escherichia coli | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0929 | - |
NADPH | at 0.15 mM aspartyl phosphate | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * 36000, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-4-aspartyl phosphate + NADPH | Arabidopsis thaliana | - |
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9FVC4 | - |
- |
Purification (Comment) | Organism |
---|---|
using anionic exchange chromatography on DEAE EMD 650 column and Q-Sepharose column | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26 | - |
- |
Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
-80°C, no noticeable loss of activity | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-4-aspartyl phosphate + NADPH | - |
Arabidopsis thaliana | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
L-4-aspartyl phosphate + NADPH + H+ | - |
Arabidopsis thaliana | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
r | |
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
Arabidopsis thaliana | L-4-aspartyl phosphate + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36000, SDS-PAGE | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.6 | - |
L-4-aspartyl phosphate | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Arabidopsis thaliana |