Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
L-aspartate 4-semialdehyde | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000 | Escherichia coli |
77000 | - |
sucrose density gradient centrifugation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K-12 | - |
Purification (Comment) | Organism |
---|---|
using heat treatment, column chromatography on DEAE-cellulose, ammonium sulfate precipitation and column chromatography on Sephadex G-200 | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.002 | - |
K12 6201 strain, enriched media plus diaminopimelic acid | Escherichia coli |
0.12 | - |
B AC70R1 strain, enriched media | Escherichia coli |
0.4 | - |
K12 23631 strain, minimal media plus N-formyllysine | Escherichia coli |
0.94 | - |
B AC70R1 strain, minimal media | Escherichia coli |
1.8 | - |
K12 6201/PoP126 strain, enriched media plus ampicillin | Escherichia coli |
2.7 | - |
B AC70R1/PoP12 strain, enriched media plus tetracycline | Escherichia coli |
5.3 | - |
B AC70R1/PoP12 strain, minimal media plus tetracycline | Escherichia coli |
26 | - |
K12 23631/pOP12 strain, minimal media plus N-formyllysine and tetracycline | Escherichia coli |
145 | - |
K12 23631/pOP12, after three times purification of the enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
Escherichia coli | L-4-aspartyl phosphate + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000 | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli |