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Literature summary for 1.2.1.11 extracted from
- Semi-aldehyde aspartic dehydrogenase of Saccharomyces cerevisiae: properties and regulation (1967), Eur. J. Biochem., 2, 341-348.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| H2CO3 | enhances activity 4 or 5 times in both directions | Saccharomyces cerevisiae |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | in the reverse reaction | Saccharomyces cerevisiae | |
| homocysteine | in the reverse reaction | Saccharomyces cerevisiae | |
| L-methionine | - |
Saccharomyces cerevisiae | |
| L-threonine | - |
Saccharomyces cerevisiae | |
| additional information | no feed-back inhibition by threonine or methionine | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
phosphate | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 140000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-4-aspartyl phosphate + NADPH | Saccharomyces cerevisiae | synthesis of threonine | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
| L-4-aspartyl phosphate + NADPH | Saccharomyces cerevisiae | synthesis of methionine | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using ammonium sulfate precipitation and column chromatography on Sephadex G-200, DEAE-cellulose and hydroxylapatite | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-4-aspartyl phosphate + NADPH | synthesis of threonine | Saccharomyces cerevisiae | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
| L-4-aspartyl phosphate + NADPH | synthesis of methionine | Saccharomyces cerevisiae | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
| L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
Saccharomyces cerevisiae | L-4-aspartyl phosphate + NADPH | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
in both directions | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Saccharomyces cerevisiae | |
| NADPH | - |
Saccharomyces cerevisiae | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
homocysteine | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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