BRENDA - Enzyme Database show
show all sequences of 1.2.1.11

Aspartic semialdehyde dehydrogenase (Escherichia coli K12)

Hegeman, G.D.; Cohen, G.N.; Morgan, R.; Methods Enzymol. 17A, 708-713 (1970)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
lyophilization of purified or partially purified enzyme, slight loss of activity
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
aromatic aldehydes
e.g.: benzaldehyde, weak
Escherichia coli
Borate
-
Escherichia coli
formaldehyde
-
Escherichia coli
Glutaraldehyde
-
Escherichia coli
iodoacetate
-
Escherichia coli
K+
-
Escherichia coli
additional information
not: chelating agents
Escherichia coli
N-ethylmaleimide
-
Escherichia coli
Na+
-
Escherichia coli
NH4+
-
Escherichia coli
p-hydroxymercuribenzoate
-
Escherichia coli
Tris salts
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
NADP+
-
Escherichia coli
0.2
-
L-aspartate 4-semialdehyde
-
Escherichia coli
6
-
HAsO42-
-
Escherichia coli
9
-
phosphate
-
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70000
-
gel filtration
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
K-12
-
Purification (Commentary)
Commentary
Organism
K-12, using ammonium sulfate precipitation and column chromatography on Sephadex G-200 and DEAE-Sephadex A-50
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
120
-
-
Escherichia coli
Storage Stability
Storage Stability
Organism
frozen or on ice, after dialysis, 1 month stable
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390180
Escherichia coli
L-4-aspartyl phosphate + NADPH
-
390180
Escherichia coli
-
additional information
maximum velocity with HAsO42- is 0.4 times that with HPO42-
390180
Escherichia coli
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Escherichia coli
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
20 min, crude extract, 20% loss of activity
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
NAD+: 0.1% or less the rate of reaction with NADP+
Escherichia coli
NADP+
-
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
NAD+: 0.1% or less the rate of reaction with NADP+
Escherichia coli
NADP+
-
Escherichia coli
General Stability (protein specific)
General Stability
Organism
lyophilization of purified or partially purified enzyme, slight loss of activity
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
aromatic aldehydes
e.g.: benzaldehyde, weak
Escherichia coli
Borate
-
Escherichia coli
formaldehyde
-
Escherichia coli
Glutaraldehyde
-
Escherichia coli
iodoacetate
-
Escherichia coli
K+
-
Escherichia coli
additional information
not: chelating agents
Escherichia coli
N-ethylmaleimide
-
Escherichia coli
Na+
-
Escherichia coli
NH4+
-
Escherichia coli
p-hydroxymercuribenzoate
-
Escherichia coli
Tris salts
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
NADP+
-
Escherichia coli
0.2
-
L-aspartate 4-semialdehyde
-
Escherichia coli
6
-
HAsO42-
-
Escherichia coli
9
-
phosphate
-
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70000
-
gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
K-12, using ammonium sulfate precipitation and column chromatography on Sephadex G-200 and DEAE-Sephadex A-50
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
120
-
-
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
frozen or on ice, after dialysis, 1 month stable
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390180
Escherichia coli
L-4-aspartyl phosphate + NADPH
-
390180
Escherichia coli
-
additional information
maximum velocity with HAsO42- is 0.4 times that with HPO42-
390180
Escherichia coli
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Escherichia coli
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
20 min, crude extract, 20% loss of activity
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Escherichia coli
Other publictions for EC 1.2.1.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741540
Dahal
Structure of a fungal form of ...
Aspergillus fumigatus, Aspergillus fumigatus ATCC MYA-4609, no activity in Homo sapiens
Acta Crystallogr. Sect. F
73
36-44
2017
-
1
1
1
-
-
-
-
-
-
-
2
-
6
-
-
1
-
-
-
-
-
2
2
-
-
-
-
-
-
-
2
-
-
-
-
1
1
2
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
3
3
-
-
-
741702
Oogai
Lysine and threonine biosynth ...
Staphylococcus aureus, Staphylococcus aureus MW2
Appl. Environ. Microbiol.
82
6150-6157
2016
-
-
-
-
1
-
-
-
-
-
-
2
-
9
-
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-
-
-
-
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2
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2
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2
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1
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2
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2
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-
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-
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-
-
2
2
-
-
-
742287
Xu
Mutagenesis of key residues i ...
Escherichia coli
ChemBioChem
17
56-64
2016
-
2
1
-
6
-
-
6
-
-
-
1
-
3
-
-
1
-
-
-
-
-
2
-
1
-
-
6
1
-
-
5
-
-
-
-
2
1
5
-
6
-
-
-
-
6
-
-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
6
1
-
-
-
-
1
1
-
6
6
743196
Subramani
-
Molecular docking and dynamic ...
Vibrio anguillarum
Lett. Drug Design Disc.
13
255-261
2016
-
-
-
-
-
-
1
-
-
-
-
-
-
1
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1
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-
-
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-
741535
Dahal
Structure of a fungal form of ...
Cryptococcus neoformans var. neoformans, Cryptococcus neoformans var. neoformans ATCC MYA-565
Acta Crystallogr. Sect. F
71
1365-1371
2015
-
1
1
1
-
-
-
-
-
-
1
2
-
5
-
-
1
-
-
-
-
-
2
2
-
-
-
-
1
-
-
2
-
-
-
-
1
1
2
1
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
2
2
-
-
-
-
1
-
-
-
-
3
3
-
-
-
742070
Thangavelu
Elaboration of a fragment lib ...
Streptococcus pneumoniae, Vibrio cholerae
Bioorg. Med. Chem.
23
6622-6631
2015
-
2
-
-
-
-
65
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
4
63
-
-
-
2
-
4
-
-
-
-
65
63
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
-
4
4
-
-
-
742704
Meng
Identification and validation ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Int. J. Mol. Sci.
16
23572-23586
2015
-
-
-
-
1
-
-
-
-
-
-
2
-
164
-
-
-
-
-
-
-
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2
-
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2
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2
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1
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2
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-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
723830
Vyas
Structures of ternary complexe ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Acta Crystallogr. Sect. D
68
671-679
2012
-
-
1
1
-
-
-
-
-
-
-
2
-
7
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
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-
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-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
724553
Luniwal
Molecular docking and enzymati ...
Streptococcus pneumoniae, Vibrio cholerae
Bioorg. Med. Chem.
20
2950-2956
2012
-
-
2
-
-
-
54
-
-
-
-
2
-
5
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
26
-
-
-
-
2
2
-
-
-
-
54
26
-
-
-
-
2
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725219
Viola
The catalytic machinery of a k ...
Candida albicans, Escherichia coli, Haemophilus influenzae, Methanocaldococcus jannaschii, Streptococcus pneumoniae, Vibrio cholerae
J. Amino Acids
2011
352538
2011
-
-
-
-
8
-
-
-
-
-
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6
-
7
-
-
-
-
-
-
-
-
12
6
-
-
-
-
-
-
-
12
-
-
-
-
-
-
12
-
8
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
12
6
-
-
-
-
-
-
-
-
-
6
6
-
-
-
725944
Evitt
Synthesis and evaluation of co ...
Salmonella enterica
Mol. Biosyst.
7
1564-1575
2011
-
-
1
-
-
-
4
3
-
-
1
2
-
1
-
-
1
-
-
-
-
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2
1
-
-
-
-
-
-
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1
-
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1
1
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-
4
-
3
-
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1
2
-
-
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1
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2
1
-
-
-
-
-
-
-
-
-
-
-
-
2
2
710717
Arachea
Expansion of the aspartate bet ...
Candida albicans
Acta Crystallogr. Sect. D
66
205-212
2010
-
-
1
1
-
-
-
-
-
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1
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1
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1
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-
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1
1
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1
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1
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1
1
1
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1
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1
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
726268
Santander
The aspartate-semialdehyde deh ...
Edwardsiella ictaluri, Edwardsiella ictaluri 93-146
PLoS ONE
5
e15944
2010
-
-
-
-
-
-
-
-
-
-
-
2
-
10
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
684158
Viola
The structure of a redundant e ...
Vibrio cholerae
Acta Crystallogr. Sect. D
D64
321-330
2008
-
1
1
1
-
-
-
-
-
-
-
1
-
5
-
-
1
-
-
-
-
-
2
1
-
-
-
-
1
-
-
1
-
-
-
-
1
1
1
1
-
-
-
-
-
-
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-
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1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
684192
Vyas
Purification, crystallization ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Acta Crystallogr. Sect. F
64
167-170
2008
-
-
1
1
-
-
-
-
-
-
3
3
-
8
-
-
1
-
-
-
-
-
4
2
-
-
-
-
-
-
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1
-
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2
1
2
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4
3
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2
-
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4
2
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-
688468
Singh
Molecular modelling and compar ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Model.
14
249-263
2008
-
-
-
-
-
-
-
-
-
-
-
1
-
9
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
1
-
-
-
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-
1
-
-
-
-
-
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-
-
-
1
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687340
Cahyanto
Construction of Lactobacillus ...
Lactobacillus plantarum, Lactobacillus plantarum IAM 12477
J. Appl. Microbiol.
102
674-679
2007
-
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670165
Cahyanto
Regulation of aspartokinase, a ...
Lactobacillus plantarum, Lactobacillus plantarum NCIMB 8826
Microbiology
152
105-112
2006
-
1
1
-
-
-
1
-
-
-
-
2
-
7
-
-
-
-
-
1
2
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
1
1
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
674667
Faehnle
Examination of key intermediat ...
Streptococcus pneumoniae
J. Biol. Chem.
281
31031-31040
2006
-
1
1
1
-
-
-
1
-
-
-
1
-
5
-
-
1
-
-
-
-
-
2
1
1
-
-
1
1
-
-
3
-
-
-
-
1
1
3
1
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
2
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
655821
Shafiani
Cloning and characterization o ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Appl. Microbiol.
98
832-838
2005
-
2
1
-
-
-
-
3
-
-
1
2
-
9
-
-
1
-
-
-
1
-
4
1
1
-
-
1
1
-
-
2
-
-
-
-
2
1
2
-
-
-
-
-
-
3
-
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673074
Cox
Design, synthesis and analysis ...
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A new branch in the family: st ...
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1
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Petrosamine B, an inhibitor of ...
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654103
Blanco
The role of substrate-binding ...
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Acta Crystallogr. Sect. D
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2004
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654110
Blanco
Critical catalytic functional ...
Haemophilus influenzae
Acta Crystallogr. Sect. D
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Faehnle
Structural basis for discrimin ...
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Acta Crystallogr. Sect. D
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2004
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654891
Alvarez
L-cystine inhibits aspartate-b ...
Escherichia coli
Biochim. Biophys. Acta
1696
23-29
2004
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High-resolution structures rev ...
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Cloning, characterization and ...
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Blanco
Capture of an intermediate in ...
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Blanco
A structural basis for the mec ...
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390202
Moore
Expression and purification of ...
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25
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Paris
Overproduction, purification, ...
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Hadfield
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Oxyanion specificity of L-Aspa ...
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Thomas
Structure of the HOM2 gene of ...
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Cloning and expression of Thio ...
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Aspartate-semialdehyde dehydro ...
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Chatterjee
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Holland
Purification and characterizat ...
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Biochemistry
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Holland
Chemical reactivity at the cat ...
Saccharomyces cerevisiae
Biochemistry
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390198
Holland
Adenosine 5-triphosphate induc ...
Saccharomyces cerevisiae
Biochemistry
12
2270-2275
1973
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1
5
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1
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1
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1
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5
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1
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390180
Hegeman
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Aspartic semialdehyde dehydrog ...
Escherichia coli
Methods Enzymol.
17A
708-713
1970
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1
12
4
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1
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1
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1
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1
1
2
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1
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1
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1
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2
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2
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1
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12
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4
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1
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1
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1
1
2
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1
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1
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1
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390186
Jenkins
Studies of a homoserineless br ...
Neurospora crassa
Biochim. Biophys. Acta
141
287-295
1967
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2
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2
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1
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1
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2
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2
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2
1
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2
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1
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1
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2
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2
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2
1
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390187
Surdin
Semi-aldehyde aspartic dehydro ...
Saccharomyces cerevisiae
Eur. J. Biochem.
2
341-348
1967
1
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5
1
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1
2
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1
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1
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1
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3
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1
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2
1
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1
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2
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5
1
1
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1
2
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1
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1
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3
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1
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390188
Black
Aspartic beta-semialdehyde deh ...
Saccharomyces cerevisiae
J. Biol. Chem.
213
39-50
1955
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1
4
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1
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1
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1
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1
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3
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3
1
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1
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1
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1
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4
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1
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1
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1
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3
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3
1
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