Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | the enzyme activity is increased about 10fold in the presence of 0.045 M (NH4)2SO4 | Thermus thermophilus | |
Na2SO4 | - |
Thermus thermophilus | |
NaCI | - |
Thermus thermophilus | |
NH4Cl | 25fold activation in the presence of 0.1 M | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | strong inhibitor | Thermus thermophilus | |
Cu2+ | strong inhibitor | Thermus thermophilus | |
Hg2+ | strong inhibitor | Thermus thermophilus | |
Monoiodoacetamide | - |
Thermus thermophilus | |
monoiodoacetate | potent inhibitor | Thermus thermophilus | |
Sodium tetrathionate | potent inhibitor | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NAD+ | at pH 8.3 and 30°C | Thermus thermophilus | |
0.3 | - |
D-glyceraldehyde 3-phosphate | at pH 8.3 and 30°C | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | activates | Thermus thermophilus | |
K+ | activates | Thermus thermophilus | |
Li+ | activates | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + arsenate + NAD+ | Thermus thermophilus | - |
1-arsono-3-phospho-D-glycerate + NADH + H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and DEAE-Sephadex A-50 gel filtration | Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.11 | - |
crude extract, at pH 8.3 and 30°C | Thermus thermophilus |
5.4 | - |
purified enzyme, at pH 8.3 and 30°C | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + arsenate + NAD+ | - |
Thermus thermophilus | 1-arsono-3-phospho-D-glycerate + NADH + H+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 33500, SDS-PAGE | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
G3P dehydrogenase | - |
Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermus thermophilus |