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Literature summary for 1.2.1.107 extracted from
- Functional expression of D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic eubacterium Thermotoga maritima in Escherichia coli Authenticity and kinetic properties of the recombinant enzyme (1993), Eur. J. Biochem., 214, 43-50 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli W3CG cells | Thermotoga maritima |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenate | the enzyme is allosterically inhibited at concentrations above 10 mM | Thermotoga maritima |  |
| D-glyceraldehyde 3-phosphate | the enzyme is allosterically inhibited at concentrations above 1 mM | Thermotoga maritima | |
| L-Glyceraldehyde 3-phosphate | the enzyme is allosterically inhibited at concentrations above 1 mM | Thermotoga maritima | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
NAD+ | at pH 8.0 and 40°C | Thermotoga maritima | |
| 3.3 | - |
D-glyceraldehyde 3-phosphate | at pH 8.0 and 40°C | Thermotoga maritima | |
| 18 | - |
arsenate | at pH 8.0 and 40°C | Thermotoga maritima | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + arsenate + NAD+ | Thermotoga maritima | - |
1-arsono-3-phospho-D-glycerate + NADH + H+ | - |
? | |
| D-glyceraldehyde 3-phosphate + arsenate + NAD+ | Thermotoga maritima MSB 8 | - |
1-arsono-3-phospho-D-glycerate + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
| Thermotoga maritima MSB 8 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phenyl-Sepharose column chromatography and DEAE-cellulose column chromatography | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + arsenate + NAD+ | - |
Thermotoga maritima | 1-arsono-3-phospho-D-glycerate + NADH + H+ | - |
? | |
| D-glyceraldehyde 3-phosphate + arsenate + NAD+ | - |
Thermotoga maritima MSB 8 | 1-arsono-3-phospho-D-glycerate + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Thermotoga maritima |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-glyceraldehyde-3-phosphate dehydrogenase | - |
Thermotoga maritima |
| GAPDH | - |
Thermotoga maritima |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Thermotoga maritima | |
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