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Literature summary for 1.2.1.106 extracted from

  • Shimizu, T.; Tomita, T.; Kuzuyama, T.; Nishiyama, M.
    Crystal structure of the LysY.LysW complex from Thermus thermophilus (2016), J. Biol. Chem., 291, 9948-9959 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.106

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of LysY complexed with amino group-carrier protein LysW-gamma-aminoadipic semialdehyde and LysW-gamma-alpha-aminoadipate, respectively. In both structures, the globular domain of LysW is recognized by positively charged residues on helix 9 and the beta11-alpha10 loop of LysY through conformational changes Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0098
-
 [amino-group carrier protein LysW]-C-terminal-gamma-aminoadipic semialdehyde pH 9.5, 60°C Thermus thermophilus
0.014
-
 NADP+ pH 9.5, 60°C Thermus thermophilus
12
-
 phosphate pH 9.5, 60°C Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[amino-group carrier protein LysW]-C-terminal-gamma-(alpha-aminoadipate) phosphate + NADPH + H+ Thermus thermophilus
-
 [amino-group carrier protein LysW]-C-terminal-gamma-aminoadipic semialdehyde + phosphate + NADP+
-
 r
[amino-group carrier protein LysW]-C-terminal-gamma-aminoadipic semialdehyde + phosphate + NADP+ Thermus thermophilus
-
 [amino-group carrier protein LysW]-C-terminal-gamma-(alpha-aminoadipate) phosphate + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Thermus thermophilus O50146 cf. EC 1.2.1.103
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.1
-
 NADPH oxidation, pH 8, 60°C Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[amino-group carrier protein LysW]-C-terminal-gamma-(alpha-aminoadipate) phosphate + NADPH + H+
-
 Thermus thermophilus [amino-group carrier protein LysW]-C-terminal-gamma-aminoadipic semialdehyde + phosphate + NADP+
-
 r
[amino-group carrier protein LysW]-C-terminal-gamma-aminoadipic semialdehyde + phosphate + NADP+
-
 Thermus thermophilus [amino-group carrier protein LysW]-C-terminal-gamma-(alpha-aminoadipate) phosphate + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
lysY
-
 Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.96
-
 [amino-group carrier protein LysW]-C-terminal-gamma-aminoadipic semialdehyde pH 9.5, 60°C Thermus thermophilus
1.1
-
 phosphate pH 9.5, 60°C Thermus thermophilus
1.2
-
 NADP+ pH 9.5, 60°C Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NADH Thermus thermophilus
NADP+
-
 Thermus thermophilus
NADPH
-
 Thermus thermophilus

General Information

General Information Comment Organism
metabolism for lysine biosynthesis, amino group-carrier protein LysW-gamma-aminoadipic acid is phosphorylated by LysZ to form LysW-gamma-aminoadipic acid phosphate, which is subsequently reduced to LysW-gamma-aminoadipic semialdehyde through a reaction catalyzed by LysY Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0089
-
 phosphate pH 9.5, 60°C Thermus thermophilus
81
-
 NADP+ pH 9.5, 60°C Thermus thermophilus
98
-
 [amino-group carrier protein LysW]-C-terminal-gamma-aminoadipic semialdehyde pH 9.5, 60°C Thermus thermophilus