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Literature summary for 1.2.1.105 extracted from

  • Green, S.R.; Storey, K.B.
    Regulation of the alpha-ketoglutarate dehydrogenasecomplex during hibernation in a small mammal, the Richardsons ground squirrel (Urocitellus richardsonii) (2020), Biochim. Biophys. Acta Proteins Proteom., 1868, 140448 .
    View publication on PubMed
Search for more literature for 1.2.1.105

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 CoA overall reaction, enzyme from euthermic animals, 22°C, pH 7.8 Urocitellus richardsonii
0.026
-
 CoA overall reaction, enzyme from euthermic animals, 37°C, pH 7.8 Urocitellus richardsonii
0.028
-
 CoA overall reaction, enzyme from euthermic animals, 5°C, pH 7.8 Urocitellus richardsonii
0.065
-
 CoA overall reaction, enzyme from hibernating animals, 37°C, pH 7.8 Urocitellus richardsonii
0.067
-
 CoA overall reaction, enzyme from hibernating animals, 5°C, pH 7.8 Urocitellus richardsonii
0.075
-
 CoA overall reaction, enzyme from hibernating animals, 22°C, pH 7.8 Urocitellus richardsonii
0.09
-
 NAD+ overall reaction, enzyme from hibernating animals, 5°C, pH 7.8 Urocitellus richardsonii
0.11
-
 NAD+ overall reaction, enzyme from euthermic animals, 5°C, pH 7.8 Urocitellus richardsonii
0.17
-
 NAD+ overall reaction, enzyme from hibernating animals, 37°C, pH 7.8 Urocitellus richardsonii
0.19
-
 NAD+ overall reaction, enzyme from euthermic animals, 22°C, pH 7.8 Urocitellus richardsonii
0.25
-
 NAD+ overall reaction, enzyme from euthermic animals, 37°C, pH 7.8 Urocitellus richardsonii
0.29
-
 2-oxoglutarate overall reaction, enzyme from euthermic animals, 22°C, pH 7.8 Urocitellus richardsonii
0.29
-
 2-oxoglutarate overall reaction, enzyme from hibernating animals, 37°C, pH 7.8 Urocitellus richardsonii
0.3
-
 NAD+ overall reaction, enzyme from hibernating animals, 22°C, pH 7.8 Urocitellus richardsonii
0.32
-
 2-oxoglutarate overall reaction, enzyme from euthermic animals, 37°C, pH 7.8 Urocitellus richardsonii
0.38
-
 2-oxoglutarate overall reaction, enzyme from hibernating animals, 22°C, pH 7.8 Urocitellus richardsonii
0.4
-
 2-oxoglutarate overall reaction, enzyme from hibernating animals, 5°C, pH 7.8 Urocitellus richardsonii
0.45
-
 2-oxoglutarate overall reaction, enzyme from euthermic animals, 5°C, pH 7.8 Urocitellus richardsonii

Organism

Organism UniProt Comment Textmining
Urocitellus richardsonii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified from skeletal muscle of euthermic and hibernating ground squirrels Urocitellus richardsonii

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
 Urocitellus richardsonii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
252
-
 pH 7.8, 22°C, overall reaction of the complex Urocitellus richardsonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + CoA + NAD+
-
 Urocitellus richardsonii succinyl-CoA + CO2 + NADH + H+
-
 ?

General Information

General Information Comment Organism
physiological function enzymes purified from skeletal muscle of euthermic and hibernating ground squirrels are evaluated at 5°C, 22°C, and 37 °C. The enzyme complex from hibernator muscle at all temperatures compared with euthermic controls exhibits a decreased affinity for CoA as well as reduced activation by Ca2+ ions at 5°C from both euthermic and hibernating conditions. The E1, E2 and E3 enzymes of the complex (OGDH, DLST, DLD) all show elevated phosphotyrosine content during hibernation as well as increased ADP-ribosylation and succinylation of hibernator OGDH Urocitellus richardsonii