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Literature summary for 1.2.1.105 extracted from

  • Aevarsson, A.; Chuang, J.L.; Wynn, R.M.; Turley, S.; Chuang, D.T.; Hol, W.G.J.
    Crystal structure of human branched-chain alpha-ketoacid dehydrogenase and the molecular basis of multienzyme complex deficiency in maple syrup urine disease (2000), Structure, 8, 277-291.
    View publication on PubMed
Search for more literature for 1.2.1.105

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Solanum tuberosum 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
 x * 105000, SDS-PAGE Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
 cv. romano
-

Purification (Commentary)

Purification (Comment) Organism
-
 Solanum tuberosum

Reaction

Reaction Comment Organism Reaction ID
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 the enzyme complex catalyzes the reaction : 2-oxoglutarate + CoA + NAD+--> succinyl-CoA + CO2 + NADH, the following partial reactions are catalyzed: 1. HOOC(CH2)2COCOOH + (thiamine diphosphate)-E1--> (HOOC(CH2)2 CHOH-thiamine-diphosphate)-E1 + CO2, 2. (HOOC(CH2)2CH OH-thiamine-diphosphate)-E1 + (LipS2)-E2--> (HOOC(CH)2 CO-(SLipSH))-E2 + (thiamine-diphosphate)-E1, 3. (HOOC(CH2)2CO-(SLipSH))-E2 + HSCoA--> (Lip(SH)2)-E2 + HOOC(CH2)2CO-SCoA, 4. (Lip(SH)2)-E2 + E3-FAD--> (LipS2)-E2 + reduced E3-FAD, 5. reduced E3-FAD + NAD+--> E3-FAD + NADH Solanum tuberosum
a

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activity of the 2-oxoglutarate dehydrogenase complex Solanum tuberosum

Storage Stability

Storage Stability Organism
-80°C, 2-oxoglutarate dehydrogenase complex, 5-15% loss of activity after several months Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + lipoamide
-
 Solanum tuberosum S-succinyldihydrolipoamide + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 105000, SDS-PAGE Solanum tuberosum