Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | stimulates 2-oxoglutarate dehydrogenase complex | Acinetobacter sp. | |
AMP | stimulates 2-oxoglutarate dehydrogenase complex | Acinetobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | 0.1 mM, 50% inhibition. 0.2 mM AMP completely overcomes the inhibition | Acinetobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
2-oxoglutarate | in presence of 0.2 mM AMP | Acinetobacter sp. | |
0.43 | - |
2-oxoglutarate | in presence of 0.2 M ADP | Acinetobacter sp. | |
2.5 | - |
2-oxoglutarate | in absence of added nucleotide | Acinetobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
- |
- |
Acinetobacter sp. 4B | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | the enzyme complex catalyzes the reaction : 2-oxoglutarate + CoA + NAD+--> succinyl-CoA + CO2 + NADH, the following partial reactions are catalyzed: 1. HOOC(CH2)2COCOOH + (thiamine diphosphate)-E1--> (HOOC(CH2)2 CHOH-thiamine-diphosphate)-E1 + CO2, 2. (HOOC(CH2)2CH OH-thiamine-diphosphate)-E1 + (LipS2)-E2--> (HOOC(CH)2 CO-(SLipSH))-E2 + (thiamine-diphosphate)-E1, 3. (HOOC(CH2)2CO-(SLipSH))-E2 + HSCoA--> (Lip(SH)2)-E2 + HOOC(CH2)2CO-SCoA, 4. (Lip(SH)2)-E2 + E3-FAD--> (LipS2)-E2 + reduced E3-FAD, 5. reduced E3-FAD + NAD+--> E3-FAD + NADH | Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + lipoamide | - |
Acinetobacter sp. | S-succinyldihydrolipoamide + CO2 | - |
? | |
2-oxoglutarate + lipoamide | - |
Acinetobacter sp. 4B | S-succinyldihydrolipoamide + CO2 | - |
? |