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Literature summary for 1.2.1.105 extracted from

  • Kornfeld, S.; Benziman, M.; Milner, Y.
    alpha-Ketoglutarate dehydrogenase complex of Acetobacter xylinum. Purification and regulatory properties (1977), J. Biol. Chem., 252, 2940-2947.
    View publication on PubMed
Search for more literature for 1.2.1.105

Activating Compound

Activating Compound Comment Organism Structure
AMP stimulates 2-oxoglutarate dehydrogenase complex Komagataeibacter xylinus

Inhibitors

Inhibitors Comment Organism Structure
NADH
-
 Komagataeibacter xylinus
succinyl-CoA
-
 Komagataeibacter xylinus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, 2-oxoglutarate dehydrogenase complex Komagataeibacter xylinus

Organism

Organism UniProt Comment Textmining
Komagataeibacter xylinus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2-oxoglutarate dehydrogenase complex Komagataeibacter xylinus

Reaction

Reaction Comment Organism Reaction ID
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 the enzyme complex catalyzes the reaction : 2-oxoglutarate + CoA + NAD+--> succinyl-CoA + CO2 + NADH, the following partial reactions are catalyzed: 1. HOOC(CH2)2COCOOH + (thiamine diphosphate)-E1--> (HOOC(CH2)2 CHOH-thiamine-diphosphate)-E1 + CO2, 2. (HOOC(CH2)2CH OH-thiamine-diphosphate)-E1 + (LipS2)-E2--> (HOOC(CH)2 CO-(SLipSH))-E2 + (thiamine-diphosphate)-E1, 3. (HOOC(CH2)2CO-(SLipSH))-E2 + HSCoA--> (Lip(SH)2)-E2 + HOOC(CH2)2CO-SCoA, 4. (Lip(SH)2)-E2 + E3-FAD--> (LipS2)-E2 + reduced E3-FAD, 5. reduced E3-FAD + NAD+--> E3-FAD + NADH Komagataeibacter xylinus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activity of the 2-oxoglutarate dehydrogenase complex Komagataeibacter xylinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + lipoamide
-
 Komagataeibacter xylinus S-succinyldihydrolipoamide + CO2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Komagataeibacter xylinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 7.6 2-oxoglutarate dehydrogenase complex Komagataeibacter xylinus

pH Range

pH Minimum pH Maximum Comment Organism
6.6 8.4 about 50% of maximal activity at pH 6.6 and at pH 8.4, 2-oxoglutarate dehydrogenase complex Komagataeibacter xylinus

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate required , 2-oxoglutarate dehydrogenase complex Komagataeibacter xylinus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.003
-
 succinyl-CoA
-
 Komagataeibacter xylinus
0.02
-
 NADH competitive inhibition with respect to NAD+, 2-oxoglutarate dehydrogenase complex Komagataeibacter xylinus