General Stability | Organism |
---|---|
successive freeze-thawing cycles of the 2-oxoglutarate dehydrogenase complex leads to complete inactivation | Neurospora crassa |
vigorous sonication of the 2-oxoglutarate dehydrogenase complex lead to complete inactivation | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
2-oxoglutarate | in presence of 0.0045 mM Ca2+ | Neurospora crassa | |
0.47 | - |
2-oxoglutarate | in presence of 0.0225 mM Ca2+ | Neurospora crassa | |
0.58 | - |
2-oxoglutarate | in presence of 0.0023 mM Ca2+ | Neurospora crassa | |
0.75 | - |
2-oxoglutarate | in presence of 0.07 mM Ca2+ | Neurospora crassa | |
0.8 | - |
2-oxoglutarate | without Ca2+ | Neurospora crassa | |
0.84 | - |
2-oxoglutarate | in presence of 0.0012 mM Ca2+ | Neurospora crassa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | diminishes the Km value of the 2-oxoglutarate dehydrogenase complex for 2-oxoglutarate in the physiological range, maximal activation, 35%, with 0.004 mM Ca2+ | Neurospora crassa | |
Mg2+ | maximal activation, 75%, with 5 mM | Neurospora crassa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52900 | - |
x * 52900, 2-oxoglutarate dehydrogenase subunit E1, SDS-PAGE | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2-oxoglutarate dehydrogenase complex | Neurospora crassa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | 2-oxoglutarate dehydrogenase complex consists of 3 enzymes: E1 (alpha-ketoglutarate dehydrogenase, EC 1.2.4.2), E2 (dihydrolipoyl transsuccinylase, EC 2.3.1.61), E3 (dihydrolipoyl dehydrogenase, EC 1.8.1.4) | Neurospora crassa | |
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | the enzyme complex catalyzes the reaction : 2-oxoglutarate + CoA + NAD+--> succinyl-CoA + CO2 + NADH, the following partial reactions are catalyzed: 1. HOOC(CH2)2COCOOH + (thiamine diphosphate)-E1--> (HOOC(CH2)2 CHOH-thiamine-diphosphate)-E1 + CO2, 2. (HOOC(CH2)2CH OH-thiamine-diphosphate)-E1 + (LipS2)-E2--> (HOOC(CH)2 CO-(SLipSH))-E2 + (thiamine-diphosphate)-E1, 3. (HOOC(CH2)2CO-(SLipSH))-E2 + HSCoA--> (Lip(SH)2)-E2 + HOOC(CH2)2CO-SCoA, 4. (Lip(SH)2)-E2 + E3-FAD--> (LipS2)-E2 + reduced E3-FAD, 5. reduced E3-FAD + NAD+--> E3-FAD + NADH | Neurospora crassa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity of the 2-oxoglutarate dehydrogenase complex | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + lipoamide | - |
Neurospora crassa | S-succinyldihydrolipoamide + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52900, 2-oxoglutarate dehydrogenase subunit E1, SDS-PAGE | Neurospora crassa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
2-oxoglutarate dehydrogenase complex | Neurospora crassa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | 20°C: about 35% of maximal activity, 45°C: about 20% of maximal activity, 2-oxoglutarate dehydrogenase complex | Neurospora crassa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
60 min, stable, 2-oxoglutarate dehydrogenase complex | Neurospora crassa |
21 | - |
60 min, about 65% loss of activity, 2-oxoglutarate dehydrogenase complex | Neurospora crassa |
30 | - |
60min, about 80% loss of activity, 2-oxoglutarate dehydrogenase complex | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
2-oxoglutarate dehydrogenase complex | Neurospora crassa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | pH 6.5: about 25% of maximal activity, pH 8.5: about 30% of maximal activity, 2-oxoglutarate dehydrogenase complex | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | required , 2-oxoglutarate dehydrogenase complex | Neurospora crassa |