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Literature summary for 1.2.1.104 extracted from

  • Jiang, Y.; Wang, J.; Zhang, G.; Oza, K.; Myers, L.; Holbert, M.A.; Sweitzer, S.
    Component co-expression and purification of recombinant human pyruvate dehydrogenase complex from baculovirus infected SF9 cells (2014), Protein Expr. Purif., 97, 9-16 .
    View publication on PubMed
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Application

Application Comment Organism
synthesis production of the functional pyruvate dehydrogenase complex. All components are coexpressed in the cytoplasm of baculovirus-infected SF9 cells by deletion of the mitochondrial localization signal sequences and E1a is FLAG-tagged to facilitate purification. The protein complex is purified using FLAG-M2 affinity resin, followed by Superdex 200 sizing chromatography. The E2 and E3BP components are then lipoylated in vitro. The resulting complex is over 90% pure and homogenous Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
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 Homo sapiens 5739
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Organism

Organism UniProt Comment Textmining
Homo sapiens P08559 and P11177 and O00330 and P10515 and P09622 P08559 i.e. subunit PdhA1, cf. EC 1.2.4.1, P11177 i.e. subunit PdhB1, cf. EC 1.2.4.1, O00330 i.e. subunit PdhX, P10515 i.e. subunit DlaT, cf. EC 2.3.1.12, P09622 i.e. subunit Dld, cf. EC 1.8.1.4, respectively
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Synonyms

Synonyms Comment Organism
DLAT
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 Homo sapiens
DLD
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 Homo sapiens
PdhA1
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 Homo sapiens
PdhB1
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 Homo sapiens
PdhX
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 Homo sapiens