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Literature summary for 1.2.1.104 extracted from

  • Jordan, F.; Arjunan, P.; Kale, S.; Nemeria, N.S.; Furey, W.
    Multiple roles of mobile active center loops in the E1 component of the Escherichia coli pyruvate dehydrogenase complex - linkage of protein dynamics to catalysis (2009), J. Mol. Catal. B, 61, 14-22 .
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
E401A mutant displays a modest threefold increase in Km pyruvate, and a significant reduction in kcat/Km pyruvate Escherichia coli
H407A mutation in E1, only modestly affects catalysis through the pyruvate decarboxylation step in isolated E1 (14% activity relative to parental E1), but inhibits the overall complex reaction by three orders of magnitude (0.15% activity compared to parental E1) Escherichia coli
K403A mutant displays a modest threefold increase in Km pyruvate, and a significant reduction in kcat/Km pyruvate Escherichia coli
additional information construction of a cysteine-less variant of the E1 component, onto which cysteines are substituted at selected loop positions. In the absence of ligand, the loop exists in two conformations, and the rate constant for loop movement is of the same order of magnitude as the turnover number for the enzyme under the same conditions Escherichia coli
N404A mutation leads to the greatest reduction in overall activity among the alanine-substituted variants and also greatly affects the Kd methyl acetylphosphonate Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AFG8 P0AFG8 i.e. pyruvate dehydrogenase E1 component, cf. EC 1.2.4.1
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General Information

General Information Comment Organism
physiological function the inner loop of the E1 component, i.e. residues 401-413, sequesters the active center from carboligase side reactions, assists the interaction between the E1 and the E2 components, thereby affecting the overall reaction rate of the entire multienzyme complex, and controls substrate access to the active center. Formation of the pre-decarboxylation intermediate is specifically affected by loop movement Escherichia coli