Literature summary for 1.2.1.104 extracted from

Snoep, J.L.; Teixeira de Mattos, M.J.; Starrenburg, M.J.; Hugenholtz, J.
Isolation, characterization, and physiological role of the pyruvate dehydrogenase complex and alpha-acetolactate synthase of Lactococcus lactis subsp. lactis bv. diacetylactis (1992), J. Bacteriol., 174, 4838-4841 .

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Inhibitors

Inhibitors	Comment	Organism	Structure
NADH	-	Lactococcus lactis subsp. lactis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0082	-	hydroxyethyl thiamine diphosphate	pH not specified in the publication, temperature not specified in the publication	Lactococcus lactis subsp. lactis
1	-	pyruvate	the complex displays cooperativity, Hill coefficient is 0.7, pH not specified in the publication, temperature not specified in the publication	Lactococcus lactis subsp. lactis

Organism

Organism	UniProt	Comment	Textmining
Lactococcus lactis subsp. lactis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Lactococcus lactis subsp. lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
6.6	-	the specific activities of E2 and E3 subunits in the complex are 30 and 0.36 U/mg, respectively, pH not specified in the publication, temperature not specified in the publication	Lactococcus lactis subsp. lactis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hydroxyethyl thiamine diphosphate + CoA + NAD+	-	Lactococcus lactis subsp. lactis	? + NADH	-	?
pyruvate + CoA + NAD+	-	Lactococcus lactis subsp. lactis	acetyl-CoA + CO2 + NADH	-	?

Subunits

Subunits	Comment	Organism
More	the complex consists of four subunits, i.e. E1alpha (44 kDa), E1beta (35 kDa), E2 (73 kDa), and E3 (60 kDa), SDS-PAGE	Lactococcus lactis subsp. lactis

Cofactor

Cofactor	Comment	Organism	Structure
CoA	-	Lactococcus lactis subsp. lactis
NAD+	-	Lactococcus lactis subsp. lactis

General Information

General Information	Comment	Organism
physiological function	addition of excess pure E3 from Enterococcus faecalis may substitute for loss of Lactococcus lactis E3 during purification	Lactococcus lactis subsp. lactis

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Release 2023.1 (January 2023)