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Literature summary for 1.2.1.104 extracted from
- Purification and properties of the pyruvate dehydrogenase complex from Salmonella typhimurium and formation of hybrids with the enzyme complex from Escherichia coli (1982), Biochim. Biophys. Acta, 705, 210-217 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0006 | - |
pyruvate | cooperativity is observed, Hill coefficient is 1.6, pH 7.6, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + CoA + NAD+ | Salmonella enterica subsp. enterica serovar Typhimurium | - |
acetyl-CoA + CO2 + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 41 | - |
pH 7.6, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + CoA + NAD+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | acetyl-CoA + CO2 + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| multimer | x * 100000, pyruvate dehydrogenase, x * 87000, dihydrolipoamide transacetylase (acetyl-CoA:dihydrolipoamide S-acetyltransferase, EC 2.3.1.12), and x * 56000, dihydrolipoamide dehydrogenase (NADH:lipoamide oxidoreductase, EC 1.6.4.3) components, respectively, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | complete cross-reactivity is found with antibodies directed against the pyruvate dehydrogenase complex from Escherichia coli and electron micrographs of both enzyme complexes reveal identical structures | Salmonella enterica subsp. enterica serovar Typhimurium |
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