Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
alpha and beta subunits purified from enzyme complex after gel filtration in the presence of 2 M KI followed by chromatography on hydroxyapatite in the presence of 8 M urea | Geobacillus stearothermophilus |
pyruvate dehydrogenase complex composed of EC 1.2.4.1, EC 2.3.1.12, EC 1.8.1.4 | Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
-15°C, 50 mM potassium phosphate buffer, pH 7.0, 2 mM EDTA, 0.15 mM phenylmethanesulfonylfluoride, 0.2 g/l NaN3, several months | Geobacillus stearothermophilus |