Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.104 extracted from

  • Hiromasa, Y.; Aso, Y.; Yamashita, S.
    Purification of pyruvate dehydrogenase complex from an extreme thermophile, Bacillus caldolyticus, and its thermal stability (1993), Biosci. Biotechnol. Biochem., 57, 1062-1066.
    View publication on PubMed
Search for more literature for 1.2.1.104

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.117
-
 pyruvate pH 7.0, 30°C [Bacillus] caldolyticus
1.27
-
 2-oxoisovalerate pH 7.0, 30°C [Bacillus] caldolyticus

Organism

Organism UniProt Comment Textmining
[Bacillus] caldolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of pyruvate dehydrogenase complex including pyruvate decarboxylase E1 [Bacillus] caldolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoisovalerate + CoA + NAD+
-
 [Bacillus] caldolyticus isobutanoyl-CoA + CO2 + NADH
-
 ?
pyruvate + CoA + NAD+
-
 [Bacillus] caldolyticus acetyl-CoA + CO2 + NADH
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 half-life 30 min, pyruvate decarboxylase subunit E1 [Bacillus] caldolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 [Bacillus] caldolyticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.7 10.2 pyruvate decarboxylase subunit E1 [Bacillus] caldolyticus