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Literature summary for 1.2.1.103 extracted from

  • Horie, A.; Tomita, T.; Saiki, A.; Kono, H.; Taka, H.; Mineki, R.; Fujimura, T.; Nishiyama, C.; Kuzuyama, T.; Nishiyama, M.
    Discovery of proteinaceous N-modification in lysine biosynthesis of Thermus thermophilus (2009), Nat. Chem. Biol., 5, 673-679 .
    View publication on PubMed
Search for more literature for 1.2.1.103

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.01 mM used in assay conditions Thermus thermophilus
Mg2+ 0.01 mM used in assay conditions Thermus thermophilus
Zn2+ 0.01 mM used in assay conditions Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine + NADPH + H+ Thermus thermophilus
-
 an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + phosphate + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine + NADPH + H+
-
 Thermus thermophilus an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + phosphate + NADP+
-
 r

Subunits

Subunits Comment Organism
? x * about 40000, SDS-PAGE Thermus thermophilus

Synonyms

Synonyms Comment Organism
lysY
-
 Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
ATP 20 mM used in assay conditions Thermus thermophilus
NADP+
-
 Thermus thermophilus
NADPH
-
 Thermus thermophilus
pyridoxal 5'-phosphate 5 mM used in assay conditions Thermus thermophilus