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Literature summary for 1.2.1.10 extracted from

  • Lan, E.; Ro, S.; Liao, J.
    Oxygen-tolerant coenzyme A-acylating aldehyde dehydrogenase facilitates efficient photosynthetic n-butanol biosynthesis in cyanobacteria (2013), Energy Environ. Sci., 6, 2672-2681 .
No PubMed abstract available
Search for more literature for 1.2.1.10

Cloned(Commentary)

Cloned (Comment) Organism
gene pduP, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis. Recombinant coexpression of PduP with the enzymes of the n-butanol synthesis pathway in Synechococcus elongatus strain PCC 7942 results in autotrophic n-butanol production Aeromonas hydrophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information no Michaelis-Menten behaviour Aeromonas hydrophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + NADH + H+ Aeromonas hydrophila best substrate acetaldehyde + CoA + NAD+
-
 r
butanoyl-CoA + NADH + H+ Aeromonas hydrophila
-
 butanal + CoA + NAD+
-
 r
propanoyl-CoA + NADH + H+ Aeromonas hydrophila
-
 propanal + CoA + NAD+
-
 r

Organism

Organism UniProt Comment Textmining
Aeromonas hydrophila
-
-
-

Oxidation Stability

Oxidation Stability Organism
the enzyme is oxygen-tolerant Aeromonas hydrophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7
-
 with butanoyl-CoA, pH 7.15, 30°C Aeromonas hydrophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + NADH + H+ best substrate Aeromonas hydrophila acetaldehyde + CoA + NAD+
-
 r
butanoyl-CoA + NADH + H+
-
 Aeromonas hydrophila butanal + CoA + NAD+
-
 ?
butanoyl-CoA + NADH + H+
-
 Aeromonas hydrophila butanal + CoA + NAD+
-
 r
additional information substrate chain length specificity of the enzyme is C2-C4, overview Aeromonas hydrophila ?
-
 ?
propanoyl-CoA + NADH + H+
-
 Aeromonas hydrophila propanal + CoA + NAD+
-
 r

Synonyms

Synonyms Comment Organism
CoA-acylating aldehyde dehydrogenase
-
 Aeromonas hydrophila
coenzyme A-acylating aldehyde dehydrogenase
-
 Aeromonas hydrophila
PduP
-
 Aeromonas hydrophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Aeromonas hydrophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.15
-
 assay at Aeromonas hydrophila

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Aeromonas hydrophila

General Information

General Information Comment Organism
metabolism the oxygen sensitivity of CoA-acylating aldehyde dehydrogenase appears to be a key limiting factor for cyanobacteria to produce alcohols through the CoA-dependent route Aeromonas hydrophila