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Literature summary for 1.2.1.10 extracted from

  • Baker, P.; Carere, J.; Seah, S.Y.
    Substrate specificity, substrate channeling, and allostery in BphJ: an acylating aldehyde dehydrogenase associated with the pyruvate aldolase BphI (2012), Biochemistry, 51, 4558-4567.
    View publication on PubMed
Search for more literature for 1.2.1.10

Cloned(Commentary)

Cloned (Comment) Organism
-
 Paraburkholderia xenovorans

Protein Variants

Protein Variants Comment Organism
C131A inactive Paraburkholderia xenovorans
C131S inactive Paraburkholderia xenovorans
D208A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Paraburkholderia xenovorans
I171A level of activation of BphI by the mutant enzyme are reduced by more than 3fold in the presence of NADH and more than 4.5fold when the enzyme is undergoing turnover. The mutation results in a 35% reduction in acetaldehyde channeling efficiency Paraburkholderia xenovorans
I171F the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme Paraburkholderia xenovorans
I195A the variant has a 20fold higher catalytic efficiency for butyraldehyde and pentaldehyde compared to the catalytic efficiency of the wild type toward its natural substrate acetaldehyde. The mutation results in a 35% reduction in acetaldehyde channeling efficiency Paraburkholderia xenovorans
I195F the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Paraburkholderia xenovorans
I195W the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme Paraburkholderia xenovorans
N170A the mutation does not substantially alter aldehyde channeling efficiencies. The level of activation of BphI by the mutant enzyme are reduced by more than 3fold in the presence of NADH and more than 4.5fold when the enzyme is undergoing turnover Paraburkholderia xenovorans
N170D level of activation of BphI by the mutant enzyme are reduced by more than 3fold in the presence of NADH and more than 4.5fold when the enzyme is undergoing turnover Paraburkholderia xenovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2
-
 picolinaldehyde mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
4.6
-
 Butyraldehyde mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
6.9
-
 picolinaldehyde mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
8.2
-
 pentaldehyde mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
12
-
 picolinaldehyde mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
12.6
-
 Butyraldehyde mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
17
-
 acetaldehyde mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
18.2
-
 picolinaldehyde wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
18.7
-
 acetaldehyde mutant enzyme D208A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
19.3
-
 Butyraldehyde mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
20.4
-
 propionaldehyde mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
23.1
-
 propionaldehyde wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
23.6
-
 acetaldehyde wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
27.2
-
 propionaldehyde mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
31.5
-
 propionaldehyde mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
31.7
-
 Butyraldehyde wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
34.9
-
 acetaldehyde mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
45.6
-
 acetaldehyde mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
79.5
-
 propionaldehyde mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
133
-
 acetaldehyde mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetaldehyde + CoA + NAD+ Paraburkholderia xenovorans
-
 acetyl-CoA + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Paraburkholderia xenovorans Q79AF6
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Paraburkholderia xenovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + CoA + NAD+
-
 Paraburkholderia xenovorans acetyl-CoA + NADH + H+
-
 ?
acetaldehyde + CoA + NAD+
-
 Paraburkholderia xenovorans acetyl-CoA + NADH + H+
-
 r
butyraldehyde + CoA + NAD+
-
 Paraburkholderia xenovorans butyryl-CoA + NADH + H+
-
 r
additional information BphJ forms a heterotetrameric complex with the class II aldolase BphI that channels aldehydes produced in the aldol cleavage reaction to the dehydrogenase via a molecular tunnel Paraburkholderia xenovorans ?
-
 ?
pentaldehyde + CoA + NAD+
-
 Paraburkholderia xenovorans pentyl-CoA + NADH + H+
-
 r
picolinaldehyde + CoA + NAD+
-
 Paraburkholderia xenovorans picolinyl-CoA + NADH + H+
-
 r
propionaldehyde + CoA + NAD+
-
 Paraburkholderia xenovorans propionyl-CoA + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
ACDH
-
 Paraburkholderia xenovorans
BphJ
-
 Paraburkholderia xenovorans
nonphosphorylating acylating aldehyde dehydrogenase
-
 Paraburkholderia xenovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.9
-
 picolinaldehyde mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
2.4
-
 picolinaldehyde mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
2.8
-
 picolinaldehyde wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
3.2
-
 picolinaldehyde mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
3.4
-
 propionaldehyde mutant enzyme I195W, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
6
-
 propionaldehyde mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
6.9
-
 acetaldehyde mutant enzyme I195W, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
7.1
-
 Butyraldehyde mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
7.6
-
 acetaldehyde mutant enzyme D208A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
7.6
-
 propionaldehyde mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
7.8
-
 acetaldehyde mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
9.5
-
 Butyraldehyde wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
12.1
-
 propionaldehyde mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
12.6
-
 Butyraldehyde mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
13
-
 pentaldehyde mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
15.4
-
 acetaldehyde mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
16.3
-
 propionaldehyde wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
16.7
-
 Butyraldehyde mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
17.2
-
 acetaldehyde wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
19.7
-
 acetaldehyde mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
24.9
-
 propionaldehyde mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
37.9
-
 acetaldehyde mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Paraburkholderia xenovorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00011
-
 picolinaldehyde mutant enzyme I195W, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.00076
-
 picolinaldehyde mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.0265
-
 pentaldehyde mutant enzyme I195W, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.0346
-
 pentaldehyde mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.0387
-
 picolinaldehyde mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.0425
-
 propionaldehyde mutant enzyme I195W, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.0433
-
 Butyraldehyde mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.0458
-
 Butyraldehyde mutant enzyme I195W, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.0728
-
 pentaldehyde mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.076
-
 pentaldehyde wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.147
-
 acetaldehyde mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.152
-
 acetaldehyde mutant enzyme I195W, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.154
-
 picolinaldehyde wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.167
-
 propionaldehyde mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.221
-
 propionaldehyde mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.223
-
 acetaldehyde mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.3
-
 Butyraldehyde wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.37
-
 Butyraldehyde mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.384
-
 propionaldehyde mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.457
-
 acetaldehyde mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.46
-
 acetaldehyde mutant enzyme D208A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.468
-
 picolinaldehyde mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.597
-
 picolinaldehyde mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.7
-
 propionaldehyde wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
0.73
-
 acetaldehyde wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
1.219
-
 propionaldehyde mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
1.323
-
 Butyraldehyde mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
1.584
-
 pentaldehyde mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
2.224
-
 acetaldehyde mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans
2.763
-
 Butyraldehyde mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) Paraburkholderia xenovorans