Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Pseudomonas sp. |
General Stability | Organism |
---|---|
activity is highest in HEPES buffer and somewhat lower in phosphate buffer, activity in Tris buffer is about half the rate in HEPES buffer | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32500 | - |
2 * 32500 + 2 * 39000, determined by nucleotide sequence, SDS-PAGE, two subunits of aldehyde dehydrogenase (acylating) and two of 4-hydroxy-2-ketovalerate | Pseudomonas sp. |
39000 | - |
2 * 32500 + 2 * 39000, determined by nucleotide sequence, SDS-PAGE, two subunits of aldehyde dehydrogenase (acylating) and two of 4-hydroxy-2-ketovalerate | Pseudomonas sp. |
148000 | - |
gel filtration, molecular mass of enzyme complex, occurs in complex with 4-hydroxy-2-ketovalerate | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + CoA + NAD+ | Pseudomonas sp. | - |
acetyl-CoA + NADH | - |
? | |
acetaldehyde + CoA + NAD+ | Pseudomonas sp. CF 600 | - |
acetyl-CoA + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. CF 600 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-80°C, 10 mM phosphate buffer, pH 7.5, 1 mM dithiothreitol | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + CoA + NAD+ | - |
Pseudomonas sp. | acetyl-CoA + NADH | - |
? | |
acetaldehyde + CoA + NAD+ | - |
Pseudomonas sp. CF 600 | acetyl-CoA + NADH | - |
? | |
formaldehyde + CoA + NAD+ | - |
Pseudomonas sp. | formyl-CoA + NADH | - |
? | |
formaldehyde + CoA + NAD+ | - |
Pseudomonas sp. CF 600 | formyl-CoA + NADH | - |
? | |
isobutyraldehyde + CoA + NAD+ | - |
Pseudomonas sp. | isobutyryl-CoA + NADH | - |
? | |
isobutyraldehyde + CoA + NAD+ | - |
Pseudomonas sp. CF 600 | isobutyryl-CoA + NADH | - |
? | |
n-butyraldehyde + CoA + NAD+ | - |
Pseudomonas sp. | n-butyryl-CoA + NADH | - |
? | |
n-butyraldehyde + CoA + NAD+ | - |
Pseudomonas sp. CF 600 | n-butyryl-CoA + NADH | - |
? | |
propanal + CoA + NAD+ | initial rate of reaction with propanal is 2.7fold slower than that with acetaldehyde | Pseudomonas sp. | propionyl-CoA + NADH | - |
? | |
propanal + CoA + NAD+ | initial rate of reaction with propanal is 2.7fold slower than that with acetaldehyde | Pseudomonas sp. CF 600 | propionyl-CoA + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 32500 + 2 * 39000, determined by nucleotide sequence, SDS-PAGE, two subunits of aldehyde dehydrogenase (acylating) and two of 4-hydroxy-2-ketovalerate | Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | activity increases gradually over pH range from 6.5 to 8.5 | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | - |
Pseudomonas sp. | |
NAD+ | - |
Pseudomonas sp. | |
NADH | rate of reaction with NADP+ is only 7% of that with NAD+ | Pseudomonas sp. |