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Literature summary for 1.2.1.10 extracted from
- Steady-state and pre-steady-state kinetics of coenzyme A linked aldehyde dehydrogenase from Escherichia coli (1981), Biochemistry, 20, 7494-7501.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | reduces lag phase before attainment of steady state rate in conjunction with NAD+ | Escherichia coli |  |
| 3-pyridinecarboxaldehyde adenine dinucleotide | NAD+ analogue, activates by binding strongly to activator site, binds weakly to catalytic site | Escherichia coli | |
| NAD+ | reduces lag phase before attainment of steady state rate in conjunction with 2-mercaptoethanol | Escherichia coli | |
General Stability
| General Stability | Organism |
|---|---|
| labile during purification, stabilization by 2-hydroxyethyldisulfide | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP-ribose | - |
Escherichia coli | |
| AMP | - |
Escherichia coli | |
| benzaldehyde | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
acetyl-CoA | - |
Escherichia coli | |
| 0.008 | - |
CoA | - |
Escherichia coli | |
| 0.025 | - |
NADH | - |
Escherichia coli | |
| 0.08 | - |
NAD+ | - |
Escherichia coli | |
| 10 | - |
acetaldehyde | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + CoA + NAD+ | Escherichia coli | during glucose fermentation | acetyl-CoA + NADH | - |
r | |
| acetaldehyde + CoA + NAD+ | Escherichia coli B / ATCC 11303 | during glucose fermentation | acetyl-CoA + NADH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH + H+ | bi-uni-uni-uni-ping-pong mechanism | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.1 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + CoA + NAD+ | - |
Escherichia coli | acetyl-CoA + NADH | - |
? | |
| acetaldehyde + CoA + NAD+ | during glucose fermentation | Escherichia coli | acetyl-CoA + NADH | - |
r | |
| acetaldehyde + CoA + NAD+ | - |
Escherichia coli B / ATCC 11303 | acetyl-CoA + NADH | - |
? | |
| acetaldehyde + CoA + NAD+ | during glucose fermentation | Escherichia coli B / ATCC 11303 | acetyl-CoA + NADH | - |
r | |
| additional information | not benzaldehyde | Escherichia coli | ? | - |
? | |
| additional information | not benzaldehyde | Escherichia coli B / ATCC 11303 | ? | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| CoA | - |
Escherichia coli | |
| NAD+ | - |
Escherichia coli | |
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