Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
NADPH | pH 7.5, 30°C | Escherichia coli | |
0.0068 | - |
reduced flavodoxin | pH 7.5, 30°C | Escherichia coli | |
0.0176 | - |
ferricytochrome c2 | pH 7.5, 30°C | Escherichia coli | |
0.0236 | - |
ferricyanide | pH 7.5, 30°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27648 | - |
- |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli HMS174 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recpmbinant protein. FLDR is bright yellow in its oxidized form and it is converted to a neutral blue semiquinone by the addition of one reducing equivalent | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADPH | - |
Escherichia coli | 2 ferrocyanide + NADP+ + H+ | - |
? | |
2 ferricyanide + NADPH | - |
Escherichia coli HMS174 | 2 ferrocyanide + NADP+ + H+ | - |
? | |
2 ferricytochrome c2 + NADPH | - |
Escherichia coli | 2 ferrocytochrome c2 + NADP+ + H+ | - |
? | |
2 ferricytochrome c2 + NADPH | - |
Escherichia coli HMS174 | 2 ferrocytochrome c2 + NADP+ + H+ | - |
? | |
additional information | the electron-transfer route is NADPH to FLDR to flavodoxin. The midpoint reduction potentials of the oxidized/semiquinone and semiquinone/hydroquinone couples of FLDR are 2308 mV and 2268 mV, respectively. Binding of 2'-adenosine monophosphate increases the midpoint reduction potentials for both FLDR couples | Escherichia coli | ? | - |
? | |
additional information | the electron-transfer route is NADPH to FLDR to flavodoxin. The midpoint reduction potentials of the oxidized/semiquinone and semiquinone/hydroquinone couples of FLDR are 2308 mV and 2268 mV, respectively. Binding of 2'-adenosine monophosphate increases the midpoint reduction potentials for both FLDR couples | Escherichia coli HMS174 | ? | - |
? | |
reduced flavodoxin + NADP+ | - |
Escherichia coli | oxidized flavodoxin + NADPH + H+ | - |
? | |
reduced flavodoxin + NADP+ | - |
Escherichia coli HMS174 | oxidized flavodoxin + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27620, calculated, 1 * 27 648, electrospray mass spectroscopy | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
FLDR | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.35 | - |
ferricytochrome c2 | pH 7.5, 30°C | Escherichia coli | |
26.8 | - |
ferricyanide | pH 7.5, 30°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | oxidized FLDR has flavin absorbance maxima at 456 nm and 400 nm, with a shoulder on the longer wavelength band at 483 nm | Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | isoelectric focusing | - |
4.8 |
Escherichia coli | calculated | - |
6.2 |