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Literature summary for 1.19.1.1 extracted from

  • Bittel, C.; Tabares, L.C.; Armesto, M.; Carrillo, N.; Cortez, N.
    The oxidant-responsive diaphorase of Rhodobacter capsulatus is a ferredoxin (flavodoxin)-NADP(H) reductase (2003), FEBS Lett., 553, 408-412.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
flavodoxin cytochrome c reductase activity of FPR is stimulated three times by the addition of flavodoxin Rhodobacter capsulatus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
ferricytochrome c presence of flavodoxin, pH 8.0, temperature not specified in the publication Rhodobacter capsulatus
0.08
-
NADPH substrate ferricyanide, pH 8.0, temperature not specified in the publication Rhodobacter capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
gel filtration Rhodobacter capsulatus
30138
-
-
Rhodobacter capsulatus

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus Q9L6V3 enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADPH + H+
-
Rhodobacter capsulatus 2 ferrocyanide + NADP+ + H+
-
?
2 ferricyanide + NADPH + H+
-
Rhodobacter capsulatus 37b4 2 ferrocyanide + NADP+ + H+
-
?
2 ferricytochrome c + NADPH + H+
-
Rhodobacter capsulatus 2 ferrocytochrome c + NADP+ + H+
-
?
additional information enzyme additionally shows diaphorase activity which is induced by treatment with methyl viologen Rhodobacter capsulatus ?
-
?
additional information enzyme additionally shows diaphorase activity which is induced by treatment with methyl viologen Rhodobacter capsulatus 37b4 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE, 1 * 30138, calculated Rhodobacter capsulatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
ferricytochrome c presence of flavodoxin, pH 8.0, temperature not specified in the publication Rhodobacter capsulatus
7.2
-
NADPH substrate ferricyanide, pH 8.0, temperature not specified in the publication Rhodobacter capsulatus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Rhodobacter capsulatus