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Literature summary for 1.18.6.2 extracted from

  • Dilworth, M.; Eldridge, M.; Eady, R.
    Correction for creatine interference with the direct indophenol measurement of NH3 in steady-state nitrogenase assays (1992), Anal. Biochem., 207, 6-10 .
    View publication on PubMed

Application

Application Comment Organism
analysis development of A method for removing other compounds which inhibit color development and for compensating for the interference produced by creatine. ThE method avoids time-consuming microdiffusion and routinely makes available the efficiency of ATP hydrolysis coupled to substrate reduction (ATP/2e ratio) with N2 as a reducible substrate Azotobacter chroococcum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O Azotobacter chroococcum
-
12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate
-
?
12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O Azotobacter chroococcum MCD1
-
12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate
-
?
additional information Azotobacter chroococcum the efficiency of ATP hydrolysis coupled to substrate reduction (ATP/2e ratio) is 4.87 at 30°C during the reduction of protons to H2 and 7.16 during the reduction of N2 ?
-
?
additional information Azotobacter chroococcum MCD1 the efficiency of ATP hydrolysis coupled to substrate reduction (ATP/2e ratio) is 4.87 at 30°C during the reduction of protons to H2 and 7.16 during the reduction of N2 ?
-
?

Organism

Organism UniProt Comment Textmining
Azotobacter chroococcum P15332 and P15334 and P15333 P15332 i.e. subunit VnfD, P15334 i.e. subunit VnfK, P15333 i.e. subunit VnfG
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Azotobacter chroococcum MCD1 P15332 and P15334 and P15333 P15332 i.e. subunit VnfD, P15334 i.e. subunit VnfK, P15333 i.e. subunit VnfG
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O
-
Azotobacter chroococcum 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate
-
?
12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O
-
Azotobacter chroococcum MCD1 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate
-
?
additional information the efficiency of ATP hydrolysis coupled to substrate reduction (ATP/2e ratio) is 4.87 at 30°C during the reduction of protons to H2 and 7.16 during the reduction of N2 Azotobacter chroococcum ?
-
?
additional information the efficiency of ATP hydrolysis coupled to substrate reduction (ATP/2e ratio) is 4.87 at 30°C during the reduction of protons to H2 and 7.16 during the reduction of N2 Azotobacter chroococcum MCD1 ?
-
?

Synonyms

Synonyms Comment Organism
vnfD alpha-chain Azotobacter chroococcum
vnfG delta-chain Azotobacter chroococcum
vnfK beta-chain Azotobacter chroococcum