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Literature summary for 1.18.6.1 extracted from

  • Fay, A.W.; Blank, M.A.; Lee, C.C.; Hu, Y.; Hodgson, K.O.; Hedman, B.; Ribbe, M.W.
    Characterization of isolated nitrogenase FeVco (2010), J. Am. Chem. Soc., 132, 12612-12618.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.18.6.1

Cloned(Commentary)

Cloned (Comment) Organism
strain YM68A expresses the His-tagged VFe protein, strain YM13A expresses the His-tagged MoFe protein, and strain DJ1143 expresses the His-tagged DELTAnifB MoFe protein Azotobacter chroococcum

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum in the FeMoco cofactor of the MoFe protein Azotobacter chroococcum
Vanadium in the FeVco cofactor Azotobacter chroococcum

Organism

Organism UniProt Comment Textmining
Azotobacter chroococcum
-
 contains Mo- and V-nitrogenases
-
Azotobacter chroococcum YM68A
-
 contains Mo- and V-nitrogenases
-

Purification (Commentary)

Purification (Comment) Organism
partially, extraction of FeVco in an active form by N-methylformamide, and gel filtration of the reconstituted protein, overview Azotobacter chroococcum

Renatured (Commentary)

Renatured (Comment) Organism
native enzyme is reconstituted from N-methylformamide extraction Azotobacter chroococcum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information FeVco is extracted intact, carrying with it the characteristic capacity to reduce C2H2 to C2H6 and, perhaps even more importantly, the ability to reduce N2 to NH3 Azotobacter chroococcum ?
-
 ?
additional information FeVco is extracted intact, carrying with it the characteristic capacity to reduce C2H2 to C2H6 and, perhaps even more importantly, the ability to reduce N2 to NH3 Azotobacter chroococcum YM68A ?
-
 ?

Synonyms

Synonyms Comment Organism
Mo-nitrogenase
-
 Azotobacter chroococcum
molybdenum-nitrogenase
-
 Azotobacter chroococcum
nitrogenase FeVco
-
 Azotobacter chroococcum
V-nitrogenase
-
 Azotobacter chroococcum
vanadium-nitrogenase
-
 Azotobacter chroococcum

Cofactor

Cofactor Comment Organism Structure
FeMo cofactor i.e. FeMoco cofactor, in the Mo-nitrogenase Azotobacter chroococcum
iron-vanadium cofactor i.e. FeVco cofactor, in the V-nitrogenase Azotobacter chroococcum
additional information the cofactors of the Mo- and V-nitrogenases, i.e. FeMoco and FeVco, are homologous metalccenters with distinct catalytic properties, differences in electronic properties and structural topology, overview Azotobacter chroococcum