Cloned (Comment) | Organism |
---|---|
phylogenetic analysis, overview | Klebsiella pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a chimeric enzyme NifDK/NifB-co in which the active site iron-molybdenum cofactor is replaced by NifB-co. NifB is a S-adenosyl-L-methionine radical enzyme that functions in the synthesis of NifB-co, an early precursor to FeMo-cofactor. In contrast to the NifDK protein containing FeMo-cofactor at the active site, NifB-co-containing NifDK is unable to reduce N2 into NH3 | Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | in the active site iron-molybdenum cofactor, and as 4Fe-4S cluster in the Fe protein | Klebsiella pneumoniae | |
Mo | in the active site iron-molybdenum cofactor | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae UN1217 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native NifDK by gel filtration and affinity chromatography on a NifB resin, elution as NifDK/NifB-co complex | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
comparison of ATP consumption coupled to H2 production by recombinant NifDK/NifB-co and by NifDK, overview | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate reduction specific activities of the recombinant chimeric NifDK/NifBco protein compared to those of wild-type NifDK, effect of molybdenum and homocitrate addition, overview. In contrast to the NifDK protein containing FeMo-cofactor at the active site, NifB-co-containing NifDK is unable to reduce N2 into NH3 | Klebsiella pneumoniae | ? | - |
? | |
additional information | substrate reduction specific activities of the recombinant chimeric NifDK/NifBco protein compared to those of wild-type NifDK, effect of molybdenum and homocitrate addition, overview. In contrast to the NifDK protein containing FeMo-cofactor at the active site, NifB-co-containing NifDK is unable to reduce N2 into NH3 | Klebsiella pneumoniae UN1217 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
molybdenum nitrogenase | - |
Klebsiella pneumoniae |
NifDK | - |
Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Klebsiella pneumoniae | |
iron-molybdenum cofactor | located at the active site in the MoFe protein | Klebsiella pneumoniae |
General Information | Comment | Organism |
---|---|---|
evolution | substrate specificity and evolutionary implications of a recombinant chimeric NifDK enzyme carrying NifB-co at its active site, NifDK/NifB-co, overview | Klebsiella pneumoniae |
physiological function | the conversion of N2 into NH3 is catalyzed by the nitrogenase enzyme, which is composed of two metalloproteins: NifDK, also termed dinitrogenase or MoFe protein, and NifH, also termed dinitrogenase reductase or Fe protein | Klebsiella pneumoniae |